1887

Abstract

is an anaerobic intestinal spirochaete that colonizes the large intestine of a variety of species of birds and mammals, including human beings. Colonization may result in a mild colitis and diarrhoea in a condition known as ‘intestinal spirochaetosis’. The catecholamine norepinephrine (NE), which is known to influence the behaviour of many bacterial species, may be present in the colon. The purpose of the current study was to determine whether exposure of to NE would influence its behaviour in assays that may reflect colonization potential. strain 95/1000 was used in all the assays. Addition of NE at a concentration of 0.05 mM to growing in anaerobic broth significantly increased spirochaete numbers after 4 days incubation. The effect of higher concentrations of NE was not significant. Exposure to 0.05 mM NE, but not to higher concentrations, also resulted in significantly more spirochaete cells entering capillary tubes containing 4 % porcine gastric mucin than occurred with untreated cultures. When NE was added to chemotaxis buffer in capillary tubes, significantly more spirochaetes were attracted to the buffer containing NE at 0.1, 0.5 and 1.0 mM than to buffer containing 0.05 mM NE, or when no NE was added. Exposure of cultures to 0.05 mM NE prior to incubation with Caco-2 monolayers resulted in more attachment to the monolayer than occurred with non-exposed cultures. These results show that at higher concentrations, NE acts as a chemoattractant for and at 0.05 mM it increases the spirochaete's growth rate, attraction to mucin and rate of attachment to cultured enterocytes. These activities are likely to enhance the ability of to colonize, and may be induced by conditions that increase NE concentrations in the intestinal tract, such as the stresses associated with crowding.

Keyword(s): NE, norephinephrine
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2011-02-01
2019-10-18
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References

  1. Bansal, T., Englert, D., Lee, J., Hegde, M., Wood, T. K. & Jayaraman, A. ( 2007; ). Differential effects of epinephrine, norepinephrine, and indole on Escherichia coli O157:H7 chemotaxis, colonization, and gene expression. Infect Immun 75, 4597–4607.[CrossRef]
    [Google Scholar]
  2. Bearson, B. L. & Bearson, S. M. ( 2008; ). The role of the QseC quorum-sensing sensor kinase in colonization and norepinephrine-enhanced motility of Salmonella enterica serovar Typhimurium. Microb Pathog 44, 271–278.[CrossRef]
    [Google Scholar]
  3. Brooke, C. J., Riley, T. V. & Hampson, D. J. ( 2006; ). Comparison of prevalence and risk factors for faecal carriage of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli in four Australian populations. Epidemiol Infect 134, 627–634.[CrossRef]
    [Google Scholar]
  4. Cogan, T. A., Thomas, A. O., Rees, L. E., Taylor, A. H., Jepson, M. A., Williams, P. H., Ketley, J. & Humphrey, T. J. ( 2007; ). Norepinephrine increases the pathogenic potential of Campylobacter jejuni. Gut 56, 1060–1065.[CrossRef]
    [Google Scholar]
  5. Doherty, N. C., Tobias, A., Watson, S. & Atherton, J. C. ( 2009; ). The effect of the human gut-signalling hormone, norepinephrine, on the growth of the gastric pathogen Helicobacter pylori. Helicobacter 14, 223–230.[CrossRef]
    [Google Scholar]
  6. Hampson, D. J. & Duhamel, G. E. ( 2006; ). Porcine colonic spirochetosis/intestinal spirochetosis. In Diseases of Swine, 9th edn, pp. 755–767. Edited by Straw, B. E., Zimmerman, J. J., D'Allaire, S. & Taylor, D. J.. Oxford. : Blackwell Publishing.
    [Google Scholar]
  7. Hampson, D. J. & Swayne, D. E. ( 2008; ). Avian intestinal spirochetosis. In Diseases of Poultry, 12th edn, pp. 922–940. Edited by Saif, Y. M.. Oxford. : Blackwell Publishing.
    [Google Scholar]
  8. Hughes, D. T., Clarke, M. B., Yamamoto, K., Rasko, D. A. & Sperandio, V. ( 2009; ). The QseC adrenergic signaling cascade in enterohemorrhagic E. coli (EHEC). PLoS Pathog 5, e1000553.[CrossRef]
    [Google Scholar]
  9. Kunkle, R. A., Harris, D. L. & Kinyon, J. M. ( 1986; ). Autoclaved liquid medium for propagation of Treponema hyodysenteriae. J Clin Microbiol 24, 669–671.
    [Google Scholar]
  10. Law, C. L. H., Grierson, J. M. & Stevens, S. M. B. ( 1994; ). Rectal spirochaetosis in homosexual men: the association with sexual practices, HIV infection and enteric flora. Genitourin Med 70, 26–29.
    [Google Scholar]
  11. Lyte, M. & Bailey, M. T. ( 1997; ). Neuroendocrine-bacterial interactions in a neurotoxin-induced model of trauma. J Surg Res 70, 195–201.[CrossRef]
    [Google Scholar]
  12. Margawani, K. R., Robertson, I. D., Brooke, C. J. & Hampson, D. J. ( 2004; ). Prevalence, risk factors and molecular epidemiology of Brachyspira pilosicoli in humans on the island of Bali, Indonesia. J Med Microbiol 53, 325–332.[CrossRef]
    [Google Scholar]
  13. Naresh, R. & Hampson, D. J. ( 2010; ). Attraction of Brachyspira pilosicoli to mucin. Microbiology 156, 191–197.[CrossRef]
    [Google Scholar]
  14. Naresh, R., Song, Y. & Hampson, D. J. ( 2009; ). The intestinal spirochete Brachyspira pilosicoli attaches to cultured Caco-2 cells and induces pathological changes. PLoS ONE 4, e8352.[CrossRef]
    [Google Scholar]
  15. Nelson, E. J., Tanudra, A., Chowdhury, A., Kane, A. V., Qadri, F., Calderwood, S. B., Coburn, J. & Camilli, A. ( 2009; ). High prevalence of spirochetosis in cholera patients, Bangladesh. Emerg Infect Dis 15, 571–573.[CrossRef]
    [Google Scholar]
  16. Pullinger, G. D., Carnell, S. C., Sharaff, F. F., van Diemen, P. M., Dziva, F., Morgan, E., Lyte, M., Freestone, P. P. & Stevens, M. P. ( 2010a; ). Norepinephrine augments Salmonella enterica-induced enteritis in a manner associated with increased net replication but independent of the putative adrenergic sensor kinases QseC and QseE. Infect Immun 78, 372–380.[CrossRef]
    [Google Scholar]
  17. Pullinger, G. D., van Diemen, P. M., Carnell, S. C., Davies, H., Lyte, M. & Stevens, M. P. ( 2010b; ). 6-hydroxydopamine-mediated release of norepinephrine increases faecal excretion of Salmonella enterica serovar Typhimurium in pigs. Vet Res 41, 68.[CrossRef]
    [Google Scholar]
  18. Reading, N. C., Rasko, D., Torres, A. G. & Sperandio, V. ( 2010; ). A transcriptome study of the QseEF two-component system and the QseG membrane protein in enterohaemorrhagic Escherichia coli O157:H7. Microbiology 156, 1167–1175.[CrossRef]
    [Google Scholar]
  19. Trivett-Moore, N. L., Gilbert, G. L., Law, C. L. H., Trott, D. J. & Hampson, D. J. ( 1998; ). Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis. J Clin Microbiol 36, 261–265.
    [Google Scholar]
  20. Trott, D. J., Combs, B. G., Oxberry, S. L., Mikosza, A. S. J., Robertson, I. D., Passey, M., Taime, J., Sehuko, R. & Hampson, D. J. ( 1997; ). The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea. Epidemiol Infect 119, 369–379.[CrossRef]
    [Google Scholar]
  21. Wanchanthuek, P., Bellgard, M. I., La, T., Ryan, K., Moolhuijzen, P., Chapman, B., Black, M., Schibeci, D., Hunter, A. & other authors ( 2010; ). The complete genome sequence of the pathogenic intestinal spirochete Brachyspira pilosicoli and comparison with other Brachyspira genomes. PLoS ONE 5, e11455.[CrossRef]
    [Google Scholar]
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