1887

Abstract

The tight control of autolysis by is critical for proper virulence gene expression and biofilm formation. A pair of dicistronic operons, SMU.575/574 () and SMU.1701/1700 (designated ), encode putative membrane proteins that share structural features with the bacteriophage-encoded holin family of proteins, which modulate host cell lysis during lytic infection. Analysis of and mutants revealed a role for these operons in autolysis, biofilm formation, glucosyltransferase expression and oxidative stress tolerance. Expression of was repressed during early exponential phase and was induced over 1000-fold as cells entered late exponential phase, whereas expression declined from early to late exponential phase. A two-component system encoded immediately upstream of (LytST) was required for activation of expression, but not for expression. In addition to availability of oxygen, glucose levels were revealed to affect and transcription differentially and significantly, probably through CcpA (carbon catabolite protein A). Collectively, these findings demonstrate that the Cid/Lrg system can affect several virulence traits of , and its expression is controlled by two major environmental signals, oxygen and glucose. Moreover, / expression is tightly regulated by LytST and CcpA.

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2010-10-01
2019-10-16
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