1887

Abstract

Two regulatory genes, and , have been previously identified upstream of the polyketide gene cluster involved in biosynthesis of the angucycline-like antibiotic auricin in CCM 3239. The gene encodes a protein similar to the response regulators of bacterial two-component signal transduction systems and has been shown to specifically activate expression of the auricin biosynthetic genes. The gene encodes a protein homologous to transcriptional repressors of the TetR family. Here we describe the characterization of the gene. Expression of the gene is directed by a single promoter, , which is induced just before stationary phase. Disruption of in CCM 3239 had no effect on growth and differentiation. However, the disrupted strain produced more auricin than its parental wild-type CCM 3239 strain. Transcription from the and promoters, directing expression of the first biosynthetic gene in the auricin gene cluster and the pathway-specific transcriptional activator, respectively, was increased in the CCM 3239 mutant strain. However, Aur1R was shown to bind specifically only to the promoter . This binding was abolished by the addition of auricin and/or its intermediates. The results indicate that the Aur1R regulator specifically represses expression of the gene, which encodes a pathway-specific activator of the auricin biosynthetic gene cluster in CCM 3239, and that this repression is relieved by auricin or its intermediates.

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2010-08-01
2020-01-19
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