1887

Abstract

The TTA codon-containing gene of , located upstream of , is in a DNA region syntenous with the homologous region of other genomes. Deletion of results in a medium-dependent sparse aerial mycelium formation and lack of sporulation. Clavulanic acid formation in this mutant decreases to about 10 % of the wild-type level depending on the medium, whereas its production is strongly stimulated by increasing the copy number. Quantitative transcriptional analysis indicates that expression of the clavulanic acid regulatory genes and decreases seven- and fourfold, respectively, in the Δ mutant, resulting in a large decrease in expression of genes encoding biosynthesis enzymes for the early steps of clavulanic acid formation and a smaller decrease in the expression of genes for the late steps of the pathway. An ARE box, 5′-TCTCATGGAGACATAGCGGGGCATGC-3′, is present upstream of and efficiently binds Brp protein, as shown by electrophoretic mobility shift assay (EMSA) analysis. The transcription level of is higher in the absence of Brp, as shown in Δ, suggesting a connection between expression and the -butyrolactone system in .

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2010-08-01
2019-10-14
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