1887

Abstract

is an oral spirochaete that has been strongly associated with chronic periodontitis. The bacterium exists as part of a dense biofilm (subgingival dental plaque) accreted to the tooth. To determine gene products important for persistence as a biofilm we developed a continuous-culture biofilm model and conducted a genome-wide transcriptomic analysis of biofilm and planktonic cells. A total of 126 genes were differentially expressed with a fold change of 1.5 or greater. This analysis identified the upregulation of putative prophage genes in the 35405 genome. Intact bacteriophage particles were isolated from and circular phage DNA was detected by PCR analysis. This represents the first, to our knowledge, functional bacteriophage isolated from , which we have designated φtd1. In biofilm cells there was also an upregulation of genes encoding several virulence factors, toxin–antitoxin systems and a family of putative transposases. Together, these data indicate that there is a higher potential for genetic mobility in when growing as a biofilm and that these systems are important for the biofilm persistence and therefore virulence of this bacterium.

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2010-03-01
2019-10-14
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