1887

Abstract

The locus of the plasmid pAD1 is an RNA-regulated addiction module encoding the peptide toxin Fst. Homology searches revealed that Fst belongs to a family of at least nine related peptides encoded on the chromosomes and plasmids of six different Gram-positive bacterial species. Comparison of an alignment of these peptides with the results of a saturation mutagenesis analysis indicated regions of the peptides important for biological function. Examination of the genetic context of the genes revealed that all of these peptides are encoded within -like loci with conserved features similar to pAD1 . All four family members were demonstrated to produce the expected toxin-encoding and regulatory RNA products. The locus from the plasmid pAMS1 was demonstrated to function as an addiction module and Fst was shown to be toxic to , suggesting that a plasmid-encoded module in that species is performing the same function. Thus, the pAD1-encoded locus appears to be the prototype of a family of related loci found in several Gram-positive species.

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2009-09-01
2019-12-11
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Oligonucleotide primers used for mutant construction [PDF file](39 KB)



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