1887

Abstract

Enterohaemorrhagic and enteropathogenic (EHEC and EPEC) inject a repertoire of effector proteins into host cells via a type III secretion system (T3SS) encoded by the locus of enterocyte effacement (LEE). OspG is an effector protein initially identified in that was shown to inhibit the host innate immune response. In this study, we found homologues in EHEC (mainly of serogroup O111) and in The T3SS encoded by the LEE was able to inject these different OspG homologues into host cells. Infection of HeLa cells with EHEC O111 inhibited the NF-B-dependent innate immune response via a T3SS-dependent mechanism. However, an EHEC O111 mutant was still able to inhibit NF-B p65 transfer to the nucleus in infected cells stimulated by tumour necrosis factor (TNF-). In addition, no difference in the inflammatory response was observed between wild-type EHEC O111 and the isogenic mutant in the rabbit ligated intestinal loop model. These results suggest that OspG is not the sole effector protein involved in the inactivation of the host innate immune system during EHEC O111 infection.

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2009-10-01
2019-10-15
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