1887

Abstract

has the ability to change its surface lipoprotein profiles frequently. The P35 family lipoproteins encoded by the genes are key players in this profile variation. The HF-2 genome has 38 genes that form three gene clusters. Most of these genes have an invertible promoter sequence that is responsible for the ON/OFF switching of individual gene expression. Here, we identified the recombinase that catalyses inversions of the gene promoters. We focused on two open reading frames of the HF-2 genome, namely MYPE2900 and MYPE8180, which show significant homology to the tyrosine site-specific recombinase (Tsr) family proteins. Since genetic tools for are still not developed, we cloned the MYPE2900 and MYPE8180 genes and expressed them in and . The promoter regions of the genes [ (MYPE6810) or (MYPE6630) genes] were also introduced into and cells expressing MYPE2900 or MYPE8180. Inversion of these promoters occurred in the presence of the MYPE2900 gene but not in the presence of the MYPE8180 gene, indicating that the MYPE2900 gene product is the recombinase that catalyses gene promoter inversions. We used a PCR-based method to detect promoter inversion. This method also enabled us to detect inversions of 10 gene promoters in HF-2 cells. All these promoter inversions occurred at the 12 bp inverted repeat (IR) sequences flanking the promoter sequence. The consensus sequence of these IRs was proposed as TAAYNNNDATTA (Y=C or T; D=A, G or T).

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2009-04-01
2020-04-10
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