1887

Abstract

is a nitrogen-fixing soil bacterium that produces the exopolysaccharide alginate. In this report we describe the isolation and characterization of strain GG4, which carries an  : : Tn mutation resulting in alginate overproduction. The gene encodes a subunit of the Na-translocating NADH : ubiquinone oxidoreductase (Na-NQR). As expected, Na-NQR activity was abolished in mutant GG4. When this strain was complemented with the genes this activity was restored and alginate production was reduced to wild-type levels. Na-NQR may be the main sodium pump of under the conditions tested (∼2 mM Na) since no Na/H-antiporter activity was detected. Collectively our results indicate that in the lack of Na-NQR activity caused the absence of a transmembrane Na gradient and an increase in alginate production.

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2009-01-01
2021-10-27
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