1887

Abstract

The specific pH values of cellular compartments affect virtually all biochemical processes, including enzyme activity, protein folding and redox state. Accurate, sensitive and compartment-specific measurements of intracellular pH (pH) dynamics in living cells are therefore crucial to the understanding of stress response and adaptation. We used the pH-sensitive GFP derivative ‘ratiometric pHluorin’ expressed in the cytosol and in the mitochondrial matrix of growing to assess the variation in cytosolic pH (pH) and mitochondrial pH (pH) in response to nutrient availability, respiratory chain activity, shifts in environmental pH and stress induced by addition of sorbic acid. The measurement allowed accurate determination of organelle-specific pH, determining a constant pH of 7.2 and a constant pH of 7.5 in cells exponentially growing on glucose. We show that pH and pH are differentially regulated by carbon source and respiratory chain inhibitors. Upon glucose starvation or sorbic acid stress, pH decrease coincided with growth stasis. Additionally, pH and growth coincided similarly in recovery after addition of glucose to glucose-starved cultures or after recovery from a sorbic acid pulse. We suggest a relation between pH and cellular energy generation, and therefore a relation between pH and growth.

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2009-01-01
2019-11-17
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