1887

Abstract

Different bacteria change their life styles in response to specific amino acids. In (now ) KT2440, arginine acts both as an environmental and a metabolic indicator that modulates the turnover of the intracellular second messenger c-di-GMP, and expression of biofilm-related genes. The transcriptional regulator ArgR, belonging to the AraC/XylS family, is key for the physiological reprogramming in response to arginine, as it controls transport and metabolism of the amino acid. To further expand our knowledge on the roles of ArgR, a global transcriptomic analysis of KT2440 and a null mutant growing in the presence of arginine was carried out. Results indicate that this transcriptional regulator influences a variety of cellular functions beyond arginine metabolism and transport, thus widening its regulatory role. ArgR acts as positive or negative modulator of the expression of several metabolic routes and transport systems, respiratory chain and stress response elements, as well as biofilm-related functions. The partial overlap between the ArgR regulon and those corresponding to the global regulators RoxR and ANR is also discussed.

Funding
This study was supported by the:
  • MCIN/AEI/10.13039/501100011033 (Award PID2019-109372GB-I00)
    • Principle Award Recipient: MaríaAntonia Molina-Henares
  • MCIN/AEI/10.13039/501100011033 (Award PID2019-109372GB-I00)
    • Principle Award Recipient: MaríaIsabel Ramos-González
  • MCIN/AEI/10.13039/501100011033 (Award PID2019-109372GB-I00)
    • Principle Award Recipient: ManuelEspinosa-Urgel
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-03-21
2024-04-22
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