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Volume 170, Issue 1

Identification of a system for hydroxamate xenosiderophore-mediated iron transport in Open Access

Abstract

One of the mechanisms employed by the opportunistic pathogen to acquire the essential element iron is the production and release of two ferric iron chelating compounds (siderophores), ornibactin and pyochelin. Here we show that is also able to take advantage of a range of siderophores produced by other bacteria and fungi (‘xenosiderophores’) that chelate iron exclusively by means of hydroxamate groups. These include the tris-hydroxamate siderophores ferrioxamine B, ferrichrome, ferricrocin and triacetylfusarinine C, the bis-hydroxamates alcaligin and rhodotorulic acid, and the monohydroxamate siderophore cepabactin. We also show that of the 24 TonB-dependent transporters encoded by the genome, two (FhuA and FeuA) are involved in the uptake of hydroxamate xenosiderophores, with FhuA serving as the exclusive transporter of iron-loaded ferrioxamine B, triacetylfusarinine C, alcaligin and rhodotorulic acid, while both FhuA and FeuA are able to translocate ferrichrome-type siderophores across the outer membrane. Finally, we identified FhuB, a putative cytoplasmic membrane-anchored ferric-siderophore reductase, as being obligatory for utilization of all of the tested bis- and tris-hydroxamate xenosiderophores apart from alcaligin.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Burkholderia cenocepacia , hydroxamate siderophore , iron transport , periplasmic reductase , sideromycins and TonB-dependent transporter
Funding
This study was supported by the:
  • Ministry of Higher Education, Malaysia
    • Principle Award Recipient: SyakiraMohammed Hussein
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-01-08
2024-05-20
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Identification of a system for hydroxamate xenosiderophore-mediated iron transport in Burkholderia cenocepacia
Microbiology 170, 001425 (2024); https://doi.org/10.1099/mic.0.001425
/content/journal/micro/10.1099/mic.0.001425
/content/journal/micro/10.1099/mic.0.001425
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