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Abstract

is a Gram-negative emerging opportunistic pathogen often present in people with respiratory diseases such as cystic fibrosis (CF). People with CF (pwCF) experience lifelong polymicrobial infections of the respiratory mucosa. Our prior work showed that promotes persistence of in mouse respiratory infections. As is typical for environmental opportunistic pathogens, has a large genome and a high degree of genetic diversity. In this study, we evaluated the genomic content of combining short and long read sequencing to construct nearly complete genomes of 10 clinical isolates. The genomes of these isolates were then compared with all publicly available genome assemblies, and each isolate was then evaluated for colonization/persistence , both alone and in coinfection with . We found that while the overall genome size and GC content were fairly consistent between strains, there was considerable variability in both genome structure and gene content. Similarly, there was significant variability in colonization and persistence in experimental mouse respiratory infections in the presence or absence of . Ultimately, this study gives us a greater understanding of the genomic diversity of clinical isolates, and how this genomic diversity relates to both interactions with other pulmonary pathogens and to host disease progression. Identifying the molecular determinants of infection with can facilitate development of novel antimicrobial strategies for a highly drug-resistant pathogen.

Funding
This study was supported by the:
  • National Institute of Diabetes and Digestive and Kidney Diseases (Award P30DK072482)
    • Principle Award Recipient: NotApplicable
  • National Heart, Lung, and Blood Institute (Award T32HL134640)
    • Principle Award Recipient: W.Edward Swords
  • Cystic Fibrosis Foundation (Award RDP R15RO)
    • Principle Award Recipient: MelissaS. McDaniel
  • Cystic Fibrosis Foundation (Award CFF SWORDS20G0)
    • Principle Award Recipient: W.Edward Swords
  • Cystic Fibrosis Foundation (Award CFF SWORDS1810)
    • Principle Award Recipient: W.Edward Swords
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article.
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2023-11-09
2024-12-14
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