1887

Abstract

Genetic screens are a key tool for linking phenotype and genotype. Transposon mutagenesis was one of the first genetic methodologies to associate genetic loci with phenotypes. The advent of next-generation sequencing transformed the use of this technique allowing rapid interrogation of whole genomes for genes that correlate with phenotype. One method is transposon directed insertion-site sequencing (TraDIS). Here we describe the method, recent developments in technology, and the advantages and disadvantages of this method compared to other genetic screening tools.

Keyword(s): bacteria , genetics , genotype , mutant , phenotype and TraDIS
Funding
This study was supported by the:
  • Australian Research Council
    • Principle Award Recipient: IanHenderson
  • University of Queensland
    • Principle Award Recipient: IanHenderson
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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/content/journal/micro/10.1099/mic.0.001385
2023-11-01
2024-05-09
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