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Volume 169, Issue 1

The NspS-MbaA system affects biofilm formation, exopolysaccharide production and motility in response to specific polyamines Open Access

Abstract

We previously showed that specific polyamines (PAs) present in the extracellular environment markedly affect extracellular polysaccharide (EPS) production, biofilm formation and motility in Rm8530. We hypothesized that extracellular PA signals were sensed and transduced by the NspS and MbaA proteins, respectively, which are homologs of the PA-sensing, c-di-GMP modulating NspS-MbaA proteins described in . Here we show that the decrease in biofilm formation and EPS production in the quorum-sensing (QS)-deficient wild-type strain 1021 in cultures containing putrescine or spermine did not occur in a 1021 mutant (1021 nspS). The transcriptional expression of in strain 1021 was significantly increased in cultures containing either of these polyamines, but not by exogenous cadaverine, 1,3-diaminopropane (DAP), spermidine (Spd) or norspermidine (NSpd). Cell aggregation in liquid cultures did not differ markedly between strain 1021 and 1021 nspS in the presence or absence of PAs. The QS-proficient Rm8530 wild-type and mutant (Rm8530 nspS) produced similar levels of biofilm under control conditions and 3.2- and 2.2-fold more biofilm, respectively, in cultures with NSpd, but these changes did not correlate with EPS production. Cells of Rm8530 nspS aggregated from two- to several-fold more than the wild-type in cultures without PAs or in those containing Spm. NSpd, Spd and DAP differently affected swimming and swarming motility in strains 1021 and Rm8530 and their respective mutants. transcription in strain Rm8530 was greatly reduced by exogenous Spm. Bioinformatic analysis revealed similar secondary structures and functional domains in the MbaA proteins of and , while their NspS proteins differed in some residues implicated in polyamine recognition in the latter species. NspS-MbaA homologs occur in a small subset of soil and aquatic bacterial species that commonly interact with eukaryotes. We speculate that the NspS-MbaA system modulates biofilm formation, EPS production and motility in response to environmental or host plant-produced PAs.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , cyclic di-GMP , exopolysaccharides , motility , NspS-MbaA , polyamine sensing and S. meliloti
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-01-30
2024-05-17
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The Sinorhizobium meliloti NspS-MbaA system affects biofilm formation, exopolysaccharide production and motility in response to specific polyamines
Microbiology 169, 001293 (2023); https://doi.org/10.1099/mic.0.001293
/content/journal/micro/10.1099/mic.0.001293
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