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Volume 169, Issue 1

Transcriptional profiling mature single- and dual-species biofilms in response to meropenem Open Access

Abstract

is a Gram-negative opportunistic pathogen frequently isolated from chronic infections of the cystic fibrosis lung and burn wounds, and is a major cause of antimicrobial-resistant nosocomial infections. is frequently co-isolated with the opportunistic fungal pathogen with the presence of in dual-species biofilms promoting tolerance to meropenem. Here, transcription profiling of mature single- or dual-species biofilms was carried out to understand the molecular mechanism(s) by which enhances meropenem tolerance. appeared to have a mild impact on the transcriptome of mature biofilms, with most differentially regulated genes being involved in interkingdom interactions (i.e. quorum sensing and phenazine biosynthesis). The addition of meropenem to mature single- or dual-species biofilms resulted in a significant bacterial transcriptional response, including the induction of the beta-lactamase, , genes involved in biofilm formation. elicited a similar transcriptional response to meropenem in the presence of , but promoted the expression of additional efflux pumps, which could play roles in increasing the tolerance of to meropenem.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Candida albicans , dual-species biofilms , meropenem , Pseudomonas aeruginosa and transcriptional profiling
Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/M02623X/1)
    • Principle Award Recipient: JessicaM. A Blair
  • Biotechnology and Biological Sciences Research Council (Award BB/R00966X/1 and BB/R00966X/2)
    • Principle Award Recipient: RebeccaA Hall
  • Medical Research Council (Award MR/L00903X/1)
    • Principle Award Recipient: RebeccaA Hall
  • Wellcome Trust (Award 108876/Z/15/Z)
    • Principle Award Recipient: FarhanaAlam
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-01-23
2024-05-17
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Transcriptional profiling of Pseudomonas aeruginosa mature single- and dual-species biofilms in response to meropenem
Microbiology 169, 001271 (2023); https://doi.org/10.1099/mic.0.001271
/content/journal/micro/10.1099/mic.0.001271
/content/journal/micro/10.1099/mic.0.001271
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