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Volume 168, Issue 9

Coordinated expression of acetyl CoA synthetase and the operon enzymes in in preparation for adaptation to acetate Free

Abstract

Successful adaptation of to constant environmental challenges demands the operation of a wide range of regulatory control mechanisms, some of which are global, while others are specific. Here, we show that the ability of acetate-negative phenotype strains of devoid of acetate kinase (AK) and phosphotransacetylase (PTA) to assimilate acetate when challenged at the end of growth on acetogenic substrates is explicable by the co-expression of acetyl CoA-synthetase (AcCoA-S) and acetate permease (AP). Furthermore, mRNA transcript measurements for , together with the enzymatic activities of their corresponding enzymes, acetyl CoA synthetase (AcCoA-S) and isocitrate lyase (ICL), clearly demonstrate that the expression of the two enzymes is inextricably linked and triggered in response to growth rate threshold signal (0.4 h 0.03: n4). Interestingly, further restriction of carbon supply to the level of starvation led to the repression of (AcCoA-S), (AK) and (PTA). Further, we provide evidence that the reaction sequence catalysed by PTA, AK and AcCoA-S is not in operation at low growth rates and that the reaction catalysed by AcCoA-S is not merely an ATP-dissipating reaction but rather advantageous, as it elevates the available free energy (Δ°) in central metabolism. Moreover, the transcriptomic data reinforce the view that the expression of PEP carboxykinase is essential in gluconeogenic phenotypes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ace operon , acetyl CoA synthetase , coordinated expression , Escherichia coli , futile cycles , isocitrate dehydrogenase and moiety conserved cycles
© 2022 The Authors
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2022-09-01
2024-05-09
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Coordinated expression of acetyl CoA synthetase and the ace operon enzymes in Escherichia coli in preparation for adaptation to acetate
Microbiology 168, 001230 (2022); https://doi.org/10.1099/mic.0.001230
/content/journal/micro/10.1099/mic.0.001230
/content/journal/micro/10.1099/mic.0.001230
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