1887

Abstract

is an important global pathogen. We were interested in understanding the role of Rv0233, a proposed subunit of the class IB ribonucleotide reductase, and its role in surviving stress conditions. We constructed an in-frame, unmarked deletion strain of and characterized its growth and survival under replicating or non-replicating conditions. We confirmed previous studies that found that Rv0233 is not essential for aerobic growth or survival in the presence of nitrite. We demonstrated that the deletion of Rv0233 does not affect susceptibility to frontline tuberculosis drugs or hydrogen peroxide. The deletion strain survived equally well under nutrient starvation or in hypoxia and was not attenuated for growth in macrophages.

Funding
This study was supported by the:
  • European Commission (Award LSHP-CT-2005-018923)
    • Principle Award Recipient: TanyaParish
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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/content/journal/micro/10.1099/mic.0.001199
2022-09-23
2024-11-13
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References

  1. Gordon SV, Parish T. Microbe Profile: Mycobacterium tuberculosis: Humanity’s deadly microbial foe. Microbiology (Reading) 2018; 164:437–439 [View Article]
    [Google Scholar]
  2. Global tuberculosis report 2021 [Internet]; 2021 https://www.who.int/publications-detail-redirect/9789240037021 accessed 17 January 2022
  3. Mowa MB, Warner DF, Kaplan G, Kana BD, Mizrahi V. Function and regulation of class I ribonucleotide reductase-encoding genes in mycobacteria. J Bacteriol 2009; 191:985–995 [View Article]
    [Google Scholar]
  4. Andersson CS, Högbom M. A Mycobacterium tuberculosis ligand-binding Mn/Fe protein reveals a new cofactor in a remodeled R2-protein scaffold. Proc Natl Acad Sci U S A 2009; 106:5633–5638 [View Article]
    [Google Scholar]
  5. Högbom M, Stenmark P, Voevodskaya N, McClarty G, Gräslund A et al. The radical site in chlamydial ribonucleotide reductase defines a new R2 subclass. Science 2004; 305:245–248 [View Article]
    [Google Scholar]
  6. Jiang W, Yun D, Saleh L, Barr EW, Xing G et al. A manganese(IV)/iron(III) cofactor in Chlamydia trachomatis ribonucleotide reductase. Science 2007; 316:1188–1191 [View Article]
    [Google Scholar]
  7. Parish T, Stoker NG. Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement. Microbiology (Reading) 2000; 146 (Pt 8):1969–1975 [View Article]
    [Google Scholar]
  8. Parish T, Roberts G, Laval F, Schaeffer M, Daffé M et al. Functional complementation of the essential gene fabG1 of Mycobacterium tuberculosis by Mycobacterium smegmatis fabG but not Escherichia coli fabG. J Bacteriol 2007; 189:3721–3728 [View Article]
    [Google Scholar]
  9. Sirgel FA, Wiid IJF, van Helden PD. Measuring minimum inhibitory concentrations in mycobacteria. Methods Mol Biol 2009; 465:173–186 [View Article] [PubMed]
    [Google Scholar]
  10. Wayne LG. In vitro model of hypoxically induced nonreplicating persistence of Mycobacterium tuberculosis. Methods Mol Med 2001; 54:247–269 [View Article]
    [Google Scholar]
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