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Volume 168, Issue 9

Rv0233 is not essential for the survival of in stress conditions Open Access

Abstract

is an important global pathogen. We were interested in understanding the role of Rv0233, a proposed subunit of the class IB ribonucleotide reductase, and its role in surviving stress conditions. We constructed an in-frame, unmarked deletion strain of and characterized its growth and survival under replicating or non-replicating conditions. We confirmed previous studies that found that Rv0233 is not essential for aerobic growth or survival in the presence of nitrite. We demonstrated that the deletion of Rv0233 does not affect susceptibility to frontline tuberculosis drugs or hydrogen peroxide. The deletion strain survived equally well under nutrient starvation or in hypoxia and was not attenuated for growth in macrophages.

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Keyword(s): essentiality , macrophage infection , Mycobacterium tuberculosis , non-replicating , ribonucleotide reductase , Rv0233 and stress conditions
Funding
This study was supported by the:
  • European Commission (Award LSHP-CT-2005-018923)
    • Principle Award Recipient: TanyaParish
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2022-09-23
2024-05-09
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.001199
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Rv0233 is not essential for the survival of Mycobacterium tuberculosis in stress conditions
Microbiology 168, 001199 (2022); https://doi.org/10.1099/mic.0.001199
/content/journal/micro/10.1099/mic.0.001199
/content/journal/micro/10.1099/mic.0.001199
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