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Volume 168, Issue 1

Trehalose biosynthetic gene of is regulated by in response to oxidative stress Free

Abstract

The gene of plays a positive role in oxidative stress responses and the WhcE protein interacts with SpiE. By utilizing 2D-PAGE analysis, we identified the gene to be under the control of . The transcription of , encoding trehalose 6-phosphatase, was stimulated by oxidative stress, and and were involved in diamide-mediated transcriptional stimulation. The Δ strain was created and found to be sensitive to the thiol-specific oxidant diamide, suggesting a role of the gene in stress responses. Genes located upstream of , such as NCgl2534 and , formed an operon and purified WhcE was able to bind to the promoter region of the operon (P), but the binding was only possible in the presence of the oxidant diamide. In addition, the transcriptional activation of P by WhcE was demonstrated in assays and the transcription was stimulated in cells exposed to the oxidant diamide. These findings indicate that WhcE is a transcriptional activator, and , which is involved in trehalose biosynthesis, has a role in oxidative stress responses in .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Corynebacterium glutamicum , stress response , trehalose and whiB-like gene
Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2019R1I1A3A01051388)
    • Principle Award Recipient: Heung-ShickLee
© 2022 The Authors
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2022-01-18
2024-05-12
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Trehalose biosynthetic gene otsB of Corynebacterium glutamicum is regulated by whcE in response to oxidative stress
Microbiology 168, 001131 (2022); https://doi.org/10.1099/mic.0.001131
/content/journal/micro/10.1099/mic.0.001131
/content/journal/micro/10.1099/mic.0.001131
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