1887

Abstract

Siderophores are produced by several bacteria that utilise iron in various environments. Elucidating the structure of a specific siderophore may have valuable applications in drug development. , a Gram-negative bacterium that inhabits a wide range of environments and can cause pneumonia, produces siderophores. However, the structure was unknown, and therefore, in this study, we aimed to elucidate it. We purified siderophores from cultures of K279a using preparative reversed-phase HPLC. The structure was analysed through LC-MS and H and C NMR. The results demonstrated that K279a produces 2,3-dihydroxybenzoylserine (DHBS), a monomer unit of enterobactin. We suggested the uptake of Iron(III) by the DHBS complex. DHBS production by K279a could be attributed to an incomplete enterobactin pathway. Drugs targeting DHBS synthesis could prevent infection.

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/content/journal/micro/10.1099/mic.0.001071
2021-07-19
2021-07-29
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