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Volume 167, Issue 5

, and membrane phospholipid remodelling in response to human serum Open Access

Abstract

, (Group A ; GAS) and (Group B ; GBS) are major aetiological agents of diseases in humans. The cellular membrane, a crucial site in host–pathogen interactions, is poorly characterized in streptococci. Moreover, little is known about whether or how environmental conditions influence their lipid compositions. Using normal phase liquid chromatography coupled with electrospray ionization MS, we characterized the phospholipids and glycolipids of , GAS and GBS in routine undefined laboratory medium, streptococcal defined medium and, in order to mimic the host environment, defined medium supplemented with human serum. In human serum-supplemented medium, all three streptococcal species synthesize phosphatidylcholine (PC), a zwitterionic phospholipid commonly found in eukaryotes but relatively rare in bacteria. We previously reported that utilizes the glycerophosphocholine (GPC) biosynthetic pathway to synthesize PC. Through substrate tracing experiments, we confirm that GAS and GBS scavenge lysoPC, a major metabolite in human serum, thereby using an abbreviated GPC pathway for PC biosynthesis. Furthermore, we found that plasmanyl-PC is uniquely present in the GBS membrane during growth with human serum, suggesting GBS possesses unusual membrane biochemical or biophysical properties. In summary, we report cellular lipid remodelling by the major pathogenic streptococci in response to metabolites present in human serum.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cellular membrane , lipidomics , phosphatidylcholine , phospholipids , plasmalogen and Streptococcus
Funding
This study was supported by the:
  • Cecil H. and Ida Green Chair in Systems Biology Science
    • Principle Award Recipient: KelliPalmer
  • National Institute of General Medical Sciences (Award U54GM069338)
    • Principle Award Recipient: ZiqiangGuan
  • National Institute of Allergy and Infectious Diseases (Award R56AI139105)
    • Principle Award Recipient: KelliPalmer
  • National Institute of Allergy and Infectious Diseases (Award R21AI130666)
    • Principle Award Recipient: KelliPalmer
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2021-05-13
2024-05-14
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Streptococcus pneumoniae, S. pyogenes and S. agalactiae membrane phospholipid remodelling in response to human serum
Microbiology 167, 001048 (2021); https://doi.org/10.1099/mic.0.001048
/content/journal/micro/10.1099/mic.0.001048
/content/journal/micro/10.1099/mic.0.001048
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