1887

Abstract

The gene is known to be involved in capsule formation in the basidiomycete yeast . A null mutant of , Δ, lacks a capsule around the cell wall and its acidic organelles are not stained with quinacrine. In order to clarify whether the Cap64 protein indeed maintains vacuole or vesicle acidification, so that the vesicle containing the capsule polysaccharide or DBB substrate are transported to the cell membrane side, the relationship between and intracellular transport genes and between and enzyme-secretion activity were analysed. Laccase activity was higher in the Δ strain than in the wild-type strain, and the transcriptional levels of and were also higher in the Δ strain than in the wild-type strain. The intracellular localization of the Cap64 protein was analysed by overexpressing an mCherry-tagged Cap64 and observing its fluorescence. The Cap64 protein was accumulated within cells in a patch-like manner. The quinacrine-stained cells were observed to analyse the acidified cell compartments; quinacrine was found to be accumulated in a patch-like manner, with the patches overlapping the fluorescence of CAP64-mCherry fusion protein. Quinacrine was thus accumulated in a patch-like fashion in the cells, and the mCherry-tagged Cap64 protein position was consistent with the position of quinacrine accumulation in cells. These results suggest that might be involved in intracellular acidification and vesicle secretion via exocytosis.

Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 16K00661 and 19K12389)
    • Principle Award Recipient: ShimizuYumi
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2021-06-14
2021-07-29
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