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Volume 166, Issue 12

Reclassification of the biocontrol agents BY-2 and Tu-100 as and insights into the genomic and specialized metabolite diversity of the species Open Access

Abstract

The genomes of two historical species strains isolated from the roots of oilseed rape and used routinely in PR China as biocontrol agents to suppress disease were sequenced. Average nucleotide identity (ANI) and digital DNA–DNA hybridization analyses demonstrated that they were originally misclassified as and now belong to the bacterial species . A broader ANI analysis of available genomes identified 292 genomes that were then subjected to core gene analysis and phylogenomics. Prediction and dereplication of specialized metabolite biosynthetic gene clusters (BGCs) defined the prevalence of multiple antimicrobial-associated BGCs and highlighted the natural product potential of . By defining the core and accessory antimicrobial biosynthetic capacity of the species, we offer an in-depth understanding of natural product capacity to facilitate the selection and testing of strains for use as biological control agents.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus velezensis , biocontrol , biosynthetic gene clusters and genomics
Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/S007652/1)
    • Principle Award Recipient: Eshwar Mahenthiralingam
  • Agricultural Science and Technology Innovation Program
    • Principle Award Recipient: Xiaojia Hu
  • National Natural Science Foundation of China (Award 31801311)
    • Principle Award Recipient: Xiaojia Hu
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2020-11-09
2024-05-01
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Reclassification of the biocontrol agents Bacillus subtilis BY-2 and Tu-100 as Bacillus velezensis and insights into the genomic and specialized metabolite diversity of the species
Microbiology 166, 1121 (2020); https://doi.org/10.1099/mic.0.000986
/content/journal/micro/10.1099/mic.0.000986
/content/journal/micro/10.1099/mic.0.000986
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