1887

Abstract

Upon DNA damage, Sulfolobales exhibit a global gene regulatory response resulting in the expression of DNA transfer and repair proteins and the repression of the cell division machinery. Because the archaeal DNA damage response is still poorly understood, we investigated the promoters of the highly induced operon. Ups pili are involved in cellular aggregation and DNA exchange between cells. With LacS reporter gene assays we identified a conserved, non-palindromic hexanucleotide motif upstream of the core promoter elements to be essential for promoter activity. Substitution of this cis regulatory motif in the promoters resulted in abolishment of cellular aggregation and reduced DNA transfer. By screening the genome we identified a total of 214 genes harbouring the hexanucleotide motif in their respective promoter regions. Many of these genes were previously found to be regulated upon UV light treatment. Given the fact that the identified motif is conserved among and promoters, we speculate that a common regulatory mechanism is present in these two species in response to DNA-damaging conditions.

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2017-05-01
2020-01-23
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