1887

Abstract

spp. are currently considered to be emerging pathogens that can code for a carbapenemase in their chromosome. Complete genome analysis of the clinical isolate sp. Sh95 revealed that this strain is a novel species, which shares a lineage with marine isolates. Characterization of its resistome showed that it codes for genes and We propose that sp. Sh95 acts as reservoir of . Moreover, analysis of mobilome showed that it contains a novel integrative and conjugative element (ICE), named ICE. Comparative analysis between the close relatives ICEPO1 from sp. W3-18-1 and ICE SXT from showed that ICEencompassed two new regions, a type III restriction modification system and a multidrug resistance integron. The integron platform contained a novel arrangement formed by gene cassettes and and a class C-attC group II intron. Furthermore, insertion of ICE occurred at a unique target site, which correlated with the presence of a different module. Mobility of ICE was assessed and demonstrated its ability to self-transfer with high efficiency to different species of bacteria. Our results show that ICE is a self-transmissible, mobile element, which can contribute to the dissemination of antimicrobial resistance; this is clearly a threat when natural bacteria from water ecosystems, such as , act as vectors in its propagation.

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2016-08-01
2019-11-16
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