1887

Abstract

Aquatic hyphomycetes are the major microbial decomposers of plant litter in streams. We selected three aquatic hyphomycete species with different abilities to tolerate, adsorb and accumulate copper and zinc, and we investigated the effects of these metals on H-ATPase activity as well as on the levels of thiol (SH)-containing compounds. Before metal exposure, the species isolated from a metal-polluted stream ( and ) had higher levels of thiol compounds than the species isolated from a clean stream (). However, rapidly increased the levels of thiols after metal exposure, emphasizing the importance of these compounds in fungal survival under metal stress. The highest amounts of metals adsorbed to fungal mycelia were found in the most tolerant species to each metal, i.e. in exposed to copper and in exposed to zinc. Short-term (10 min) exposure to copper completely inhibited the activity of H-ATPase of and whilst zinc only led to a similar effect on . However, at longer exposure times (8 days) the most metal-tolerant species exhibited increased H-ATPase activities, suggesting that the plasma membrane proton pump may be involved in the acclimation of aquatic hyphomycetes to metals.

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2016-05-01
2022-01-20
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