1887

Abstract

Only limited information is available on the roles of different rumen ciliate community types, first described by Eadie in 1962, in enteric methane (CH) formation by their ruminant hosts. If the different types were differentially associated with CH formation, then ciliate community typing could be used to identify naturally high and low CH-emitting animals. Here we measured the CH yields [g CH (kg feed dry matter intake, DMI)] of 118 sheep fed a standard pelleted lucerne diet at two different times, at least 2 weeks apart. There were significant differences ( < 2.2 × 10, Wilcoxon rank sum test) in the CH yields ( ± ) from sheep selected as high [16.7 ± 1.5 g CH (kg DMI)] and low emitters [13.3 ± 1.5 g CH (kg DMI)]. A rumen sample was collected after each of the two measurements, and ciliate composition was analysed using barcoded 454 Titanium pyrosequencing of 18S rRNA genes. The genera found, in order of mean relative abundance, were , , , , , and , none of which was significantly correlated with the CH emissions ranking associated with the rumen sample. Ciliate communities naturally assembled into four types (A, AB, B and O), characterized by the presence and absence of key genera. There was no difference in CH yield between sheep that harboured different ciliate community types, suggesting that these did not underlie the natural variation in CH yields. Further research is needed to unravel the nature of interactions between ciliate protozoa and other rumen micro-organisms, which may ultimately lead to contrasting CH emission phenotypes.

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2016-03-01
2021-10-27
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