1887

Abstract

The E-signal is one of five intercellular signals (named A- to E-signal) guiding fruiting body development in , and it has been shown to be a combination of the branched-chain fatty acid (FA) iso-15 : 0 and the diacylmonoalkyl ether lipid TG1. Developmental mutants HB015 (Δ MXAN_4265::) and (MXAN_1528::) are blocked at different stages of fruiting body and spore formation as they cannot form the required iso-FA or the actual ether lipid, respectively. In order to define the structural basis of the E-signal, different mono- and triglycerides containing ether or ester bonds were synthesized and used for complementation of these mutants. Here, the monoalkylglyceride -1--(13-methyltetradecyl)glycerol exhibited comparably high levels of complementation in both mutants, restoring fruiting body and spore formation, identifying iso-15 : 0 -alkylglycerol, part of the natural lipid TG1, as the ‘signalophore’ of E-signalling.

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2016-01-01
2019-12-12
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References

  1. Ahrendt T., Wolff H., Bode H. B.. 2015; [CrossRef] Neutral and phospholipids of Myxococcus xanthus lipidome during fruiting body formation and germination. Appl Environ Microbiol81:6538–6547
    [Google Scholar]
  2. Bhat S., Ahrendt T., Dauth C., Bode H. B., Shimkets L. J.. 2014; Two lipid signals guide fruiting body development of Myxococcus xanthus. MBio5: e00939-13 [CrossRef][PubMed]
    [Google Scholar]
  3. Bode H. B., Ring M. W., Schwär G., Kroppenstedt R. M., Kaiser D., Müller R.. 2006; 3-Hydroxy-3-methylglutaryl-coenzyme A (CoA) synthase is involved in biosynthesis of isovaleryl-CoA in the myxobacterium Myxococcus xanthus during fruiting body formation. J Bacteriol188:6524–6528 [CrossRef][PubMed]
    [Google Scholar]
  4. Bode H. B., Ring M. W., Schwär G., Altmeyer M. O., Kegler C., Jose I. R., Singer M., Müller R.. 2009; Identification of additional players in the alternative biosynthesis pathway to isovaleryl-CoA in the myxobacterium Myxococcus xanthus. ChemBioChem10:128–140 [CrossRef][PubMed]
    [Google Scholar]
  5. Bretscher A. P., Kaiser D.. 1978; Nutrition of Myxococcus xanthus, a fruiting myxobacterium. J Bacteriol133:763–768[PubMed]
    [Google Scholar]
  6. Downward J., Toal D.. 1995; Branched-chain fatty acids: the case for a novel form of cell-cell signalling during Myxococcus xanthus development. Mol Microbiol16:171–175 [CrossRef][PubMed]
    [Google Scholar]
  7. Dworkin M., Gibson S. M.. 1964; A system for studying microbial morphogenesis: rapid formation of microcysts in Myxococcus xanthus. Science146:243–244 [CrossRef][PubMed]
    [Google Scholar]
  8. Garcia R., Pistorius D., Stadler M., Müller R.. 2011; Fatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 fatty acids. J Bacteriol193:1930–1942 [CrossRef][PubMed]
    [Google Scholar]
  9. Garza A. G., Harris B. Z., Greenberg B. M., Singer M.. 2000; Control of asgE expression during growth and development of Myxococcus xanthus. J Bacteriol182:6622–6629 [CrossRef][PubMed]
    [Google Scholar]
  10. Hoiczyk E., Ring M. W., McHugh C. A., Schwär G., Bode E., Krug D., Altmeyer M. O., Lu J. Z., Bode H. B.. 2009; Lipid body formation plays a central role in cell fate determination during developmental differentiation of Myxococcus xanthus. Mol Microbiol74:497–517 [CrossRef][PubMed]
    [Google Scholar]
  11. Kaiser D., Welch R.. 2004; Dynamics of fruiting body morphogenesis. J Bacteriol186:919–927 [CrossRef][PubMed]
    [Google Scholar]
  12. Komano T., Inouye S., Inouye M.. 1980; Patterns of protein production in Myxococcus xanthus during spore formation induced by glycerol, dimethyl sulfoxide, and phenethyl alcohol. J Bacteriol144:1076–1082[PubMed]
    [Google Scholar]
  13. Kroos L., Kuspa A., Kaiser D.. 1986; A global analysis of developmentally regulated genes in Myxococcus xanthus. Dev Biol117:252–266 [CrossRef][PubMed]
    [Google Scholar]
  14. Kuspa A., Kaiser D.. 1989; Genes required for developmental signalling in Myxococcus xanthus: three asg loci. J Bacteriol171:2762–2772[PubMed]
    [Google Scholar]
  15. Li Y., Luxenburger E., Müller R.. 2013; An alternative isovaleryl CoA biosynthetic pathway involving a previously unknown 3-methylglutaconyl CoA decarboxylase. Angew Chem Int Ed Engl52:1304–1308 [CrossRef][PubMed]
    [Google Scholar]
  16. Lorenzen W., Ahrendt T., Bozhüyük K.A.J., Bode H. B.. 2014; A multifunctional enzyme is involved in bacterial ether lipid biosynthesis. Nat Chem Biol10:425–427 [CrossRef][PubMed]
    [Google Scholar]
  17. Mahmud T., Wenzel S. C., Wan E., Wen K. W., Bode H. B., Gaitatzis N., Müller R.. 2005; A biosynthetic pathway to isovaleryl-CoA in myxobacteria: the involvement of the mevalonate pathway. ChemBioChem6:322–330 [CrossRef][PubMed]
    [Google Scholar]
  18. Müller F.-D., Treuner-Lange A., Heider J., Huntley S. M., Higgs P. I.. 2010; Global transcriptome analysis of spore formation in Myxococcus xanthus reveals a locus necessary for cell differentiation. BMC Genomics11:264 [CrossRef][PubMed]
    [Google Scholar]
  19. Rasband W. S.. 1997–2014; ImageJ Bethesda, MD: U.S. National Institutes of Health;http://rsb.info.nih.gov/ij /
    [Google Scholar]
  20. Ring M. W., Schwär G., Thiel V., Dickschat J. S., Kroppenstedt R. M., Schulz S., Bode H. B.. 2006; Novel iso-branched ether lipids as specific markers of developmental sporulation in the myxobacterium Myxococcus xanthus. J Biol Chem281:36691–36700 [CrossRef][PubMed]
    [Google Scholar]
  21. Zusman D. R., Scott A. E., Yang Z., Kirby J. R.. 2007; Chemosensory pathways, motility and development in Myxococcus xanthus. Nat Rev Microbiol5:862–872 [CrossRef][PubMed]
    [Google Scholar]
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