Thioredoxin (Trx) maintains intracellular thiol groups in a reduced state and is involved in a wide range of cellular processes, including ribonucleotide reduction, sulphur assimilation, oxidative stress responses and arsenate detoxification. The industrially important lactic acid bacterium contains two Trxs. TrxA is similar to the well-characterized Trx homologue from and contains the common WCGPC active site motif, while TrxD is atypical and contains an aspartate residue in the active site (WCGDC). To elucidate the physiological roles of the two Trx paralogues, deletion mutants Δ, Δ and ΔΔ were constructed. In general, the ΔΔ strain was significantly more sensitive than either of the Δ and Δ mutants. Upon exposure to oxidative stress, growth of the Δ strain was diminished while that of the Δ mutant was similar to the wild-type. The lack of TrxA also appears to impair methionine sulphoxide reduction. Both Δ and Δ strains displayed growth inhibition after treatment with sodium arsenate and tellurite as compared with the wild-type, suggesting partially overlapping functions of TrxA and TrxD. Overall the phenotype of the Δ mutant matches established functions of WCGPC-type Trx while TrxD appears to play a more restricted role in stress resistance of


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