A new insertion sequence (IS) was identified in the genome of the 1-haloalkane-utilizing bacterium NCIMB 13064. The insertion element is 1415 bp long, does not contain terminal inverted repeats, and is not flanked by directly repeated sequences. IS belongs to the IS family of transposable elements, and forms a separate subfamily, along with IS Two copies of IS were found in NCIMB 13064 and one, two or three copies of a similar sequence were detected in five other 1-haloalkane-degrading strains. There were no sequences homologous to IS found in the 1-haloalkane-degrading 170 and sp. HA1 or in several strains which do not utilize haloalkanes. IS was originally found in plasmid pRTL1 of NCIMB 13064, which harbours genes encoding utilization of 1-haloalkanes, and was located 5 kbp upstream of the haloalkane dehalogenase gene (). Although the second copy of IS in strain NCIMB 13064 was also present on the pRTL1 plasmid, these sequences do not apparently comprise a single composite transposon encoding haloalkane utilization. An analysis of derivatives of NCIMB 13064 revealed that IS was involved in genome rearrangements. IS appeared to change its location as a result of transposition and as a result of other rearrangements of the NCIMB 13064 genome.


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