The study of the intra-specific phylogenetic structure of is essential to understand the participation of several strains in malo-lactic fermentation (MLF). RFLP of the PCR-amplified 16S-23S rDNA intergenic spacer region (ISR) was performed in and other related species. The RFLP patterns with seven endonucleases were identical for the 37 strains, but differed from those obtained for all other species tested. This method could provide an invaluable insight for molecular identification of the wine leuconostocs. The RFLP relationships of members of the genera and were highly similar to those previously reported by 16S and 23S rRNA sequencing studies. The 16S-23S rDNA ISR was sequenced in five strains of A single tRNA was detected. The ISR sequence seems to be identical in the two rRNA () operons found in and no significant sequence variation was observed between strains that revealed relative differences as previously shown by PFGE. Results from the present study demonstrated that is phylogenetically a very homogeneous species (according to DNA-DNA hybridization studies) and sustain that this species is different from the genus The extremely conserved ISR of these organisms suggests that strains currently isolated and characterized must have spread with the transfer of viticulture rather than coming from indigenous populations.


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