The 2.4.1 gene, which encodes a phosphoribosyl AMP-cyclohydrolase that catalyses the third step in the histidine biosynthetic pathway, has been isolated from a genomic library of this phototrophic bacterium by complementation of an his mutant. Analysis of the nucleotide sequence of the gene reveals that it encodes a deduced product of 119 aa with a predicted molecular mass of 13.4 kDa. In contrast to the situation in the gene encodes a unifunctional protein and it is not linked to the gene. The absence of a single histidine operon like that of was confirmed by PFGE experiments and complementation analysis of a mutant that was constructed by marker exchange. The location of in the genome has been determined to be at map co-ordinate 2275+20 of chromosome I.


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