1887

Abstract

The 2.4.1 gene, which encodes a phosphoribosyl AMP-cyclohydrolase that catalyses the third step in the histidine biosynthetic pathway, has been isolated from a genomic library of this phototrophic bacterium by complementation of an his mutant. Analysis of the nucleotide sequence of the gene reveals that it encodes a deduced product of 119 aa with a predicted molecular mass of 13·4 kDa. In contrast to the situation in the gene encodes a unifunctional protein and it is not linked to the gene. The absence of a single histidine operon like that of was confirmed by PFGE experiments and complementation analysis of a mutant that was constructed by marker exchange. The location of in the genome has been determined to be at map co-ordinate 2275±20 of chromosome I.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-2071
1996-08-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-2071.html?itemId=/content/journal/micro/10.1099/13500872-142-8-2071&mimeType=html&fmt=ahah

References

  1. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. 1989 Current Protocols in Molecular Biology. New York: Greene Publishing Associates & Wiley- Interscience;
    [Google Scholar]
  2. Bazzicalupo M., Fani R., Gallori E., Turbantini L., Polsinelli M. 1987; Cloning of the histidine, pyrimidine, and cysteine genes of Azospirillum brasilense. Expression of pyrimidine and three clustered histidine genes in Escherichia coli . Mol Gen Genet 206:76–80
    [Google Scholar]
  3. Becker-Rudzik M., Young D.A., Marrs B.L. 1992; Sequence of the indolglycerol phosphate synthase ( trpC ) gene from Rhodobacter capsulatus . J Bacteriol 174:5482–5484
    [Google Scholar]
  4. Beckler G.S., Reeve J.N. 1986; Conservation of the primary structure in the hisI gene of the archaebacterium Methanococcus vannielii , the eubacterium E. coli and the eucaryote Saccharomyces cerevisiae . Mol Gen Genet 204:133–140
    [Google Scholar]
  5. Bibb M.J., Findlay P.R., Johnson M.W. 1984; The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequence. Gene 30:157–166
    [Google Scholar]
  6. Bruni C.B., Carlomagno M.S., Formisano S., Paolella G. 1986; Primary and secondary structural homologies between the H1S4 gene product of Saccharomyces cerevisiae and the hisIE and hisD gene products of E. coli and S. typhimurium . Mol Gen Genet 203:389–396
    [Google Scholar]
  7. Carlomagno M.S., Chiarotti L., Alifano P., Nappo A.G., Bruni C.B. 1988; Structure and function of the Salmonella typhimurium and Escherichia coli K-12 histidine operons. J Mol Biol 203:585–606
    [Google Scholar]
  8. Clark D.J., Maaløe O. 1967; DNA replication and the division cycle of E.coli . J Mol Biol 23:99–112
    [Google Scholar]
  9. Delorme C., Ehrlich S.D., Renault P. 1992; Histidine biosynthesis genes in Lactococcus lactis subsp. lactis . J Bacteriol 174:6571–6579
    [Google Scholar]
  10. Ditta G., Schmidthauser T., Yakobson E., Lu P., Liang X.V., Finlay D.R., Guiney D., Helinski D.R. 1985; Plasmids related to the broad host range vector pRK290 useful for gene cloning and for monitoring gene expression. Plasmid 13:149–153
    [Google Scholar]
  11. Donhaue T.F., Parabaugh P.J., Fink G.R. 1982; The nucleotide sequence of the HIS4 region of yeast. Gene 18:47–59
    [Google Scholar]
  12. Dower J.W., Miller F.J., Ragsdale W.C. 1988; High efficiency transformation of E.coli by high voltage electroporation. Nucleic Acids Res 16:6127–6145
    [Google Scholar]
  13. Fani R., Alifano P., Allotta G., Bazzicalupo M., Carlomagno M.S., Gallori E., Rivellini F., Polsinelli M. 1993; The histidine operon of Azospirillum brasilense : organization, nucleotide sequence and functional analysis. Res Microbiol 144:187–200
    [Google Scholar]
  14. Fellay R., Frey J., Krisch H. 1987; Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52:147–154
    [Google Scholar]
  15. Gibson J., Stackenbrandt E., Zablen L.B., Gupta R., Woese C.R. 1979; A phylogenetic analysis of the purple photosynthetic bacteria. Curr Microbiol 3:59–64
    [Google Scholar]
  16. Goldschmidt E.P., Cater M.S., Matney T.S., Butler M.A., Greeve A. 1970; Genetic analysis of the histidine operon in E.coli K12. Genetics 66:219–229
    [Google Scholar]
  17. Hanahan D. 1983; Studies on transformation of E.coli with plasmids. J Mol Biol 166:557–580
    [Google Scholar]
  18. Hartman P.E., Hartman Z., Stahl R.C., Ames B. 1971; Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella . Adv Genet 16:1–34
    [Google Scholar]
  19. Henner D.J., Band L., Flaggs G., Chen E. 1986; The organization and nucleotide sequence of the Bacillus subtilis hisH , tyrA end aroE genes. Gene 49:147–152
    [Google Scholar]
  20. Lang H.P., Cogdell R.J., Takaichi S., Hunter C.N. 1995; Complete DNA sequence, specific Tn 5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides . J Bacteriol 177:2064–2073
    [Google Scholar]
  21. Legerton T.L., Yanofsky C. 1986; Cloning and characterization of the multifunctional his3 gene of Neurospora crassa . Gene 39:129–140
    [Google Scholar]
  22. Limauro D., Avitabile A., Cappeliano C., Puglia A.M., Bruni C.B. 1990; Cloning and characterization of the histidine biosynthetic gene cluster of Streptomyces coelicolor A3(2). Gene 90:31–41
    [Google Scholar]
  23. Mackenzie C., Chidambaram M., Sodergren E.J., Kaplan S., Weinstock G.M. 1995; DNA repair mutants of R. sphaeroides . J Bacteriol 177:3027–3035
    [Google Scholar]
  24. Martin R.G., Berberich M.A., Ames B.N., Davis W.W., Goldberger R.F., Yourno J.D. 1971; Enzymes and intermediates of histidine biosynthesis in Salmonella typhimurium . Methods Enspymol 17B:3–44
    [Google Scholar]
  25. Miller J.H. 1992 A Short Course in Bacterial Genetics, Handbook. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. O´Byrne C.P., NÍ Bhriain N., Dorman C.J. 1992; The DNA supercoiling-sensitive expression of the Salmonella typhimurium his operon requires the his attenuator and is modulated by anaerobiosis and by osmolarity. Mol Microbiol 6:2467–2476
    [Google Scholar]
  27. Pemberton J.M., Bowen A.R.St G. 1981; High frequency chromosome transfer in Khodopseudomonas sphaeroides promoted by broad-host range plasmid RP1 carrying mercury transposon Tn 501 . J Bacteriol 147:110–117
    [Google Scholar]
  28. Pfennig N., Trupper H.G. 1983; Taxonomy of phototrophic green and purple bacteria: a review. Ann Microbiol 134:9–20
    [Google Scholar]
  29. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  31. Shine J., Dalgarno L. 1975; Determinant of cistron specificity in bacterial ribosomes. Nature 254:34–38
    [Google Scholar]
  32. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Biotechnology 11:784–791
    [Google Scholar]
  33. Smith C.L., Cantor C.R. 1987; Purification, specific fragmentation and separation of large DNA molecules. Methods Engymol 155:449–467
    [Google Scholar]
  34. Suwanto A., Kaplan S. 1989a; Physical and genetic mapping of the R.j phaeroides 2.4.1 genome: genome size, fragment identification and gene localization. J Bacteriol 171:5840–5849
    [Google Scholar]
  35. Suwanto A., Kaplan S. 1989b; Physical and genetic mapping of the R. sphaeroides 2.4.1 genome: presence of two unique circular chromosomes. J Bacteriol 171:5850–5859
    [Google Scholar]
  36. Winkler M.E. 1987; Biosynthesis of histidine. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 1: pp. 395–411 Edited by Neidhardt F.C., Ingraham J.L., Brooks Low K., Magasanik B., Schaechter M., Umbarger H.E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  37. Zinchenko V., Churin Y., Shestopalov V., Shestakov S. 1994; Nucleotide sequence and characterization of the R hodobacter sphaeroides glnB and glnA genes. Microbiology 140:2143–2151
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-2071
Loading
/content/journal/micro/10.1099/13500872-142-8-2071
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error