In the MetR and PurR proteins positively and negatively regulate gene expression, respectively. A DNase I footprint analysis showed that both proteins bind independently to the control region. The PurR protein blocks RNA polymerase (RNAP) from binding to the promoter. The presence of hypoxanthine, the co-repressor of PurR, increases the ability of PurR to prevent RNAP binding, providing a model for repression of the gene by PurR. In contrast, MetR alters the RNAP footprint pattern of the control region. In addition, the MetR footprint is increased in the presence of RNAP, suggesting that the two proteins might interact.


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