1887

Abstract

The AJ12036) gene, encoding 2-oxoglutarate dehydrogenase (E1o subunit of the 2-oxoglutarate dehydrogenase complex), has been isolated and identified as an homologous counterpart of the and genes. The nucleotide sequence of a 4394 bp chromosomal fragment containing the gene was determined. The gene comprised 3771 bp (1257 codons, including the initiation codon) and a molecular mass of 138656 Da was predicted for the OdhA polypeptide. Northern blot analysis revealed a 3·9 kb transcript. The size of the transcript, together with the presence of a rho-independent terminator-like structure, suggests that is monocistronic. Cells harbouring plasmids carrying showed a threefold increase in specific 2-oxoglutarate dehydrogenase complex activity and expression of a protein with an apparent molecular mass of 136 kDa, in good agreement with the predicted size of the OdhA polypeptide. The C-terminal region of the OdhA protein shows strong sequence similarity to E1os from other organisms. OdhA has an N-terminal extension not found in previously reported E1os. The amino acid sequence of this extension shows similarity to that of the C-terminal region of dihydrolipoamide -succinyltransferase (E2o) subunits of 2-oxoglutarate dehydrogenase complexes and dihydrolipoamide -acetyltransferase (E2p) subunits of pyruvate dehydrogenase complexes. It suggests that the gene might encode a novel bifunctional protein with E1o and E2o activities.

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1996-12-01
2021-10-17
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