1887

Abstract

Extracts from K-12 contained two distinct enzymes capable of catalysing the phosphorylation of hydroxymethylpyrimidine (HMP) to HMP monophosphate: pyridoxine kinase (EC 2.7.1.35) and an enzyme that has not previously been genetically analysed, HMP kinase (EC 2.7.1.49). Two distinct genes, and specify the activities of the former and latter enzymes, respectively. The inactivation of both genes by independent mutations in the same cell resulted in the complete loss of HMP kinase activity. Experiments with a series of strains that carry mutations in and revealed that the ability of the double mutant () to utilize HMP in thiamin pyrophosphate biosynthesis was restored by introducing the wild-type allele corresponding to the mutation. The locus was mapped on the chromosome near the and loci, which govern the activities of phosphomethylpyrimidine kinase (EC 2.7.4.7) and hydroxyethylthiazole kinase (EC 2.7.1.50), respectively.

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1996-10-01
2021-05-05
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References

  1. Adelberg E.A., Mandel M., Chen G.C. 1965; Optimal conditions for mutagenesis by N-methyl-N´-nitro-N-nitroso- guanidine in Escherichia coli K-12.. Biophys Biochem Res Commun 18:788–795
    [Google Scholar]
  2. Bachmann B.J. 1983; Linkage map of Escherichia coli K-12, 7th edn.. Bacteriol Rev 47:180–230
    [Google Scholar]
  3. Henderson H.M. 1965; The conversion of pyridoxine phosphate into pyridoxal phosphate in Escherichia coli. . Biochem J 95:775–779
    [Google Scholar]
  4. Horn P. B. V., Backstrom A. D., Stewart V., Begley T. P. 1993; Structural genes for thiamine biosynthetic enzymes (thiCEEGH) in Escherichia coli K-12.. J Bacteriol 175:982–992
    [Google Scholar]
  5. Imamura N., Nakayama H. 1981; thiD locus of Escherichia coli. . Experientia 37:1265–1266
    [Google Scholar]
  6. Imamura N., Nakayama H. 1982; thiK and thiL loci of Escherichia coli. . J Bacteriol 151:708–717
    [Google Scholar]
  7. Kawasaki T., Nakata T., Nose Y. 1968; Genetic mapping with a thiamine-requiring auxotroph of Escherichia coli K-12 defective in thiamine phosphate pyrophosphorylase.. J Bacteriol 95:1483–1485
    [Google Scholar]
  8. Lam H.-M., Winkler M.E. 1992; Characterization of the complexpdxH- tyrS operon of Escherichia coli K-12 and pleiotrophic phenotypes caused by pdxH insertion mutations.. J Bacteriol 174:6033–6045
    [Google Scholar]
  9. Lederberg J., Lederberg E.M. 1952; Replica plating and indirect selection of bacterial mutants.. J Bacteriol 63:399–406
    [Google Scholar]
  10. Lennox E.S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1.. Virology 1:190–206
    [Google Scholar]
  11. Lewin L.M., Brown G.M. 1961; The biosynthesis of thiamine. III. Mechanism of enzymatic formation of the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine.. J Biol Chem 236:2768–2771
    [Google Scholar]
  12. Low K.B. 1973; Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12 .. J Bacteriol 113:798–812
    [Google Scholar]
  13. Miller J.H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Mizote T., Nakayama H. 1989a; Purification and properties of hydroxymethylpyrimidine kinase from Escherichia coli. . Biochim Biophys Acta 991:109–113
    [Google Scholar]
  15. Mizote T., Nakayama H. 1989b; The thiM locus and its relation to phosphorylation of hydroxyethylthiazole in Escherichia coli. . J Bacteriol 171:3228–3232
    [Google Scholar]
  16. Nakayama H. 1990; Genetic analysis of thiamin pyrophosphate biosynthesis in Escherichia coli. . Vitamins 64:619–632
    [Google Scholar]
  17. Nakayama H., Hayashi R. 1972a; Biosynthesis of thiamine pyrophosphate in Escherichia coli. . J Bacteriol 109:936–938
    [Google Scholar]
  18. Nakayama H., Hayashi R. 1972b; Biosynthetic pathway of thiamine pyrophosphate: a special reference to the thiamine monophosphate-requiring mutant and the thiamine pyrophosphate-requiring mutant of Escherichia coli. . J Bacteriol 112:1118–1126
    [Google Scholar]
  19. Nakayama H., Hayashi R. 1979; Isolation and characterization of Escherichia coli mutants auxotrophic for thiamine phosphates.. Methods Enzymol 62:94–101
    [Google Scholar]
  20. Osborn M., Person S., Philips S., Funke F. 1967; A determination of mutagen specificity in bacteria using nonsense mutants of bacteriophage T4.. J Mol Biol 26:437–447
    [Google Scholar]
  21. Shimizu S., Dempsey B.W. 1976; Genetic map position of the pdxH gene in Escherichia coli. . J Bacteriol 127:1593–1594
    [Google Scholar]
  22. Taylor A.L., Trotter C.D. 1967; Revised linkage map of Escherichia coli. . Bacteriol Rev 31:332–353
    [Google Scholar]
  23. Turner J.M., Happold F. C. 1961; Pyridoxamine phosphate- oxidase and pyridoxal phosphate-phosphatase activities in Escherichia coli. . Biochem J 78:364–372
    [Google Scholar]
  24. Yamada K., Kumaoka H. 1982; Incorporation of a two-carbon fragment derived from ribose of 5-aminoimidazole ribotide into the pyrimidine moiety of thiamine.. Biochem Int 5:771–776
    [Google Scholar]
  25. Watanabe K., Isoi K., Nakayama H., Hayashi R. 1977; Some properties of a mutant strain of Escherichia coli requiring high concentration of 2-methyl-4-amino-5-hydroxymethylpyrimidine.. J Nutr Sci Vitaminol 23:81–93
    [Google Scholar]
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