1887

Abstract

A screening of putative killer yeast strains showed that spore-forming ascomycetous yeasts of the genera and displayed the broadest range of activity against sensitive strains of spp. and (NCYC 500) showed extensive anti- activity against strains isolated from clinical specimens. killer factor was produced in minimal media as a function of growth and its activity reached constant levels as cells entered stationary phase. The proteinaceous killer toxin was found to be unstable outwith a specific range of temperature and pH (above 30 °C and pH 4·0), and further analysis showed that the active toxin molecule was an acidic polypeptide with a relative molecular mass between 1·8-5·0 kDa. At critical concentrations the killer factor exerted a greater effect on stationary phase cells of than cells from an exponential phase of growth. At low concentrations, the killer toxin produced a fungistatic effect on sensitive yeasts but at higher concentrations there was evidence to suggest that membrane damage accounted for the zymocidal effects of the killer factor. The cidal nature of the toxin was reflected in a rapid decrease in sensitive cell viability. Findings presented suggest that killer toxin has potential as a novel antimycotic agent in combatting medically important strains of

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/content/journal/micro/10.1099/13500872-141-8-2003
1995-08-01
2019-11-18
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