1887

Abstract

A screening of putative killer yeast strains showed that spore-forming ascomycetous yeasts of the genera and displayed the broadest range of activity against sensitive strains of spp. and (NCYC 500) showed extensive anti- activity against strains isolated from clinical specimens. killer factor was produced in minimal media as a function of growth and its activity reached constant levels as cells entered stationary phase. The proteinaceous killer toxin was found to be unstable outwith a specific range of temperature and pH (above 30 °C and pH 4°0), and further analysis showed that the active toxin molecule was an acidic polypeptide with a relative molecular mass between 1°8-5°0 kDa. At critical concentrations the killer factor exerted a greater effect on stationary phase cells of than cells from an exponential phase of growth. At low concentrations, the killer toxin produced a fungistatic effect on sensitive yeasts but at higher concentrations there was evidence to suggest that membrane damage accounted for the zymocidal effects of the killer factor. The cidal nature of the toxin was reflected in a rapid decrease in sensitive cell viability. Findings presented suggest that killer toxin has potential as a novel antimycotic agent in combatting medically important strains of

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1995-08-01
2021-07-27
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References

  1. Ashida S., Shimazaki T., Kitano K., Hara S. 1983; New killer toxin of Hansenula mrakii. . Agric Biol Chem 47:2953–2955
    [Google Scholar]
  2. Barnett J.A., Payne R.W., Yarrow D. 1983 Yeasts: Characteristics and Identification Cambridge:: Cambridge University Press.;
    [Google Scholar]
  3. Boone C., Sdicu A.-M., Wagner J., Degre R., Sanchez C., Bussey H. 1990; Integration of the yeast K1 killer toxin gene into the genome of marked wine yeasts and its effect on vinification. Am J Enol Vitic 41:37–42
    [Google Scholar]
  4. Bortol A., Nudel C., Fraile E., de Torres R., Giuietti A., Spencer J.F.T., Spencer D. 1986; Isolation of the yeast with killer activity and its breeding with an industrial brewing strain by protoplast fusion. Appl Microbiol Biotechnol 24:414–416
    [Google Scholar]
  5. Bussey H. 1972; Effects of the yeast killer factor on sensitive cells. Nature New Biol 235:73–75
    [Google Scholar]
  6. Bussey H. 1991; K1 killer toxin, a pore-forming protein from yeast. Mol Microbiol 5:2339–2343
    [Google Scholar]
  7. Bussey H., Sherman D. 1973; Yeast killer factor: ATP leakage and coordinate inhibition of macromolecular synthesis in sensitive cells. Biochim Biophys Acta 298:868–875
    [Google Scholar]
  8. Caprilli F., Prignano G., Latella C., Tavarozzi S. 1985; Amplification of the killer systems for differentiation of Candida albicans strains. Mykosen 28:569–573
    [Google Scholar]
  9. Chan S.J., Patzelt C., Duguid J.R., Quinn P., Labrecque A., Noyes B., Keim P., Henrikson R.L., Steiner D.F. 1979; Precursor in the biosynthesis of insulin and other peptide hormones. In From Gene to Protein pp. 361–402 Russel T. R., Brew K., Faber H., Schultz J. Edited by New York:: Academic Press.;
    [Google Scholar]
  10. Dignard D., Whiteway M., Germain D., Tessier D., Thomas D.Y. 1991; Expression in yeast of a cDNA copy of the K2 killer toxin gene. Mol & Gen Genet 227:127–136
    [Google Scholar]
  11. Grafe U. 1982; Relevance of microbial nitrogen metabolism to production of secondary metabolites. InOverproduction of Microbial Products pp. 63–75 Vrumphanzl V., Sikyta B., Venek Z. Edited by London:: Academic Press.;
    [Google Scholar]
  12. Hodgson V.J. 1993 Anti-Candida activity of killer toxins from the yeast Williopsis mrakii PhD thesis, Dundee Institute of Technology; Scotland,UK.:
    [Google Scholar]
  13. Kagan B. L. 1983; Mode of action of yeast killer toxins: channel formation in lipid bilayer membranes. Nature 302:709–711
    [Google Scholar]
  14. Kandel J.S. 1988; Killer systems in pathogenic fungi. In Viruses of Fungi and Simple Eukaryotes pp. 243–263 Koltin Y., Leibowitz M. Edited by New York:: Marcel Dekker.;
    [Google Scholar]
  15. Kazantseva D.I., Zimina M.S. 1989; Yeast killer strains with a broad spectrum of activity: search of collection strains and preliminary identification. Mikrobiologiya 58:230–235
    [Google Scholar]
  16. Kimura T., Kitamoto Y., Matsuoka K., Nakamura K., limura Y., Kito Y. 1993; Isolation and nucleotide sequences of the genes encoding killer toxins from Hansenula mrakii and H. saturnus. . Gene 137:265–270
    [Google Scholar]
  17. Kotani H., Shinmyo A., Enatsu T. 1977; Killer toxin for sake yeast: properties and effects of adenosine 5-phosphate and calcium ion on killing action. J Bacteriol 129:640–650
    [Google Scholar]
  18. Kreger van Rij N.W. 1984 The Yeasts: a Taxonomic Study, 3. Amsterdam:: Elsevier Science Publications.;
    [Google Scholar]
  19. Kurtzman C.P. 1984; Synonomy of the yeast genera Hansenula and Pichia demonstrated through comparisons of deoxyribonucleic acid relatedness. Antonie Leeuwenhoek 50:209–217
    [Google Scholar]
  20. Makower M., Bevan E.A. 1963; The physiological basis of killer character in yeast. In Genetics Today Proceedings of the XI International Congress on Genetics 1 pp. 202–203 Goerts S. J. Edited by The Hague:: MacMillan.;
    [Google Scholar]
  21. Martinac B., Zhu H., Kubalski A., Zhou X., Culbertson M., Bussey H., Kung C. 1990; Yeast K1 killer toxin forms ion channels in sensitive yeast sphaeroplasts and in artificial liposomes. Proc Natl Acad Sci USA 87:6228–6232
    [Google Scholar]
  22. Meinhardt F., Kempen F., Kaemper J., Esser K. 1990; Linear plasmids among eukaryotes: fundamentals and application. Curr Genet 17:89–95
    [Google Scholar]
  23. Michalcakova S., Sulo P., Slavikova E. 1993; Killer yeast of Kluyveromyces and Hansenula genera with potential application in fermentation and therapy. Acta Biotechnol 13:341–350
    [Google Scholar]
  24. Middelbeek E.J., Hermans J.M.H., Stumm C. 1979; Production, purification and properties of a Pichia kluyveri killer toxin. Antonie Leeuwenhoek 45:437–450
    [Google Scholar]
  25. Morace G., Archibussi C., Sesito M., Polonelli L. 1984; Strain differentiation of pathogenic yeasts by the killer system. Myco pathologia 84:81–85
    [Google Scholar]
  26. Morace G., Manzara S., Dettori G., Fanti F., Conti S., Campana L., Polonelli L., Chezzi C. 1989; Biotyping of bacterial isolates using the killer system. Eur J Epidemiol 5:303–310
    [Google Scholar]
  27. Naumova T.I., Naumov G.I. 1973; Comparative genetics of yeast. XII. Study of antagonistic interrelations in Saccharomyces yeast. Genetika 9:85–90
    [Google Scholar]
  28. Ohta Y., Tsukada V., Sugimeri T. 1984; Production, purification and characterisation of HY1, an anti-yeast substance, produced by Hansenula saturnus. . Agric Biol Chem 48:903–908
    [Google Scholar]
  29. O’Leary E. 1988; Secretion of multiple killer toxins by Williopsis mrakii. . Abstracts of the Annual Meeting of the American Society for Microbiology F-19:394
    [Google Scholar]
  30. Ouchi K., Kawase N., Nakano S., Akiyama H. 1978; Stabilisation of the yeast killer factor by glycerol. Agric Biol Chem 42:1407–1417
    [Google Scholar]
  31. de la Pena P., Barros F., Gascon S., Ramos S., Lazo P.S. 1980; Primary effects of yeast killer toxin. Biochem Biophys Res Commun 96:544–550
    [Google Scholar]
  32. Pfeiffer P., Radler F. 1982; Purification and characterization of the protein toxin from Saccharomyces cerevisiae strain 28. J Gen Microbiol 128:2699–2706
    [Google Scholar]
  33. Philliskirk G., Young T.W. 1975; The occurrence of killer character in yeast of various genera. Antonie Leeuwenhoek 41:147–151
    [Google Scholar]
  34. Pietras D.F., Bruenn J.A. 1976; The molecular biology of yeast killer factor. Int J Biochem 7:173–179
    [Google Scholar]
  35. Polonelli L, Morace G. 1986; Reevaluation of the killer phenomenon. J Clin Microbiol 24:866–869
    [Google Scholar]
  36. Polonelli L., Archibussi C., Sestitio M., Morace G. 1983; Killer system: a simple method for differentiation of Candida albicans strains. J Clin Microbiol 17:774–780
    [Google Scholar]
  37. Polonelli L., Lorenzini R., De Bernadis F., Morace G. 1986; Potential therapeutic effect of yeast killer toxin. Mycopathologia 96:103–107
    [Google Scholar]
  38. Polonelli L., Conti S., Campani L., Morace G., Fanti F. 1989; Yeast killer toxins and dimorphism. J Clin Microbiol 27:1423–1425
    [Google Scholar]
  39. Sawant A.D., Abdelal A.T., Ahear D.G. 1989; Purification and characterization of the anti-Candida toxin of Pichia anomala WC65. Antimicrob Agents Chemother 33:48–52
    [Google Scholar]
  40. Skipper N., Bussey J. 1977; Mode of action of yeast toxins: energy requirement for Saccharomyces cerevisiae killer toxin. J Bacteriol 129:668–677
    [Google Scholar]
  41. Somers J.M., Bevan E.A. 1969; The inheritance of killer character in yeast. Genet Res Commun 13:71–83
    [Google Scholar]
  42. Sossin W.S., Fisher J.M., Scheller R.H. 1989; Cellular and molecular biology of neuropeptide processing and packaging. Neuron 2:1407–1417
    [Google Scholar]
  43. Stark M.J.R., Boyd A., Mileham A.J., Romanos M.A. 1990; The plasmid-encoded killer system of Kluyveromyces lactis. . Yeast 6:1–29
    [Google Scholar]
  44. Starmer W.T., Ganter P.F., Aberdeen V. 1987; The ecological role of killer yeasts in natural communities of yeasts. Can J Microbiol 33:783–796
    [Google Scholar]
  45. Stumm C., Hermans J.M.H., Middlebeek E.J., Croes A.F., De Vries G.J.M. 1977; Killer sensitive relationships in yeasts from natural habitats. Antonie Leeuwenhoek 43:125–128
    [Google Scholar]
  46. Tipper D.J., Bostian K.A. 1984; Double-stranded ribonucleic acid killer systems in yeasts. Microbiol Rev 48:125–126
    [Google Scholar]
  47. Walker G.M., MacLeod A.M., Hodgson V.J. 1995; Interactions between killer yeasts and pathogenic fungi. FEMS Microbiol Lett 127:213–222
    [Google Scholar]
  48. white J.H., Butler A.R., stark M.J.R. 1989; Kluyveromyces lactis toxin does not inhibit adenylate cyclase. Nature 341:666–668
    [Google Scholar]
  49. Woods D.R. 1966 Studies of the nature of ‘killer factor’ in yeast PhD thesis, University of Oxford; UK:
    [Google Scholar]
  50. Woods D.R., Bevan E. A. 1968; Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. . Heredity 21:121–130
    [Google Scholar]
  51. Yamamoto T., Imai M., Tachibana K., Mayumi M. 1986a; Application of monoclonal antibodies to the isolation and characteristics of a killer toxin secreted by Hansenula mrakii. . FEBS Lett 195:253–257
    [Google Scholar]
  52. Yamamoto T., Hiratani T., Hirata M., Imai K., Yamaguchi H. 1986b; Killer toxin from Hansenula mrakii selectively inhibits cell wall synthesis in sensitive yeast. FEBS Lett 197:50–54
    [Google Scholar]
  53. Young T.W. 1982; The properties and brewing performance of brewing yeasts possessing killer character. J Amer Soc Brew Chem 42:1–4
    [Google Scholar]
  54. Young T.W., Yagui M. 1978; A comparison of the killer character in different yeasts and its classification. Antonie Leeuwenhoek 44:59–77
    [Google Scholar]
  55. Zhu H., Bussey H., Thomas D.Y., Gagnon J., Bell A.W. 1987; Determination of the carboxyl termini of the α and β subunits of the yeast K1 killer toxin. J Biol Chem 262:10728–10732
    [Google Scholar]
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