1887

Abstract

A strain that forms effective nodules on soybean () and alfalfa () is described. The strain, 042B, was isolated from root nodules of alfalfa in Xinjiang Autonomous Region of China. Experiments showed that strain 042B was able to nodulate soybean as effectively as USDA110, a widely used inoculant strain. Under hydroponic conditions, both strains performed similarly in many respects such as time required for the appearance of nodules, total nitrogenase activity, plant top dry weight and total plant nitrogen. In soil pot experiments, total nitrogen, seed weight and seed nitrogen of soybean plants inoculated with strains 042B or USDA110 were much higher than those of the uninoculated control, but there were no differences between plants with strain 042B relative to strain USDA110. However, when strain 042B was the inoculant, the number of nodules was greater, but the nodules were smaller than those of strain USDA110. Results from ELISA showed that nodule occupancy of strain 042B in soybean ranged from 82-90% and that of strain USDA110 ranged from 78-86%. Strain 042B can grow in the presence of 5% (w/v) NaCl, at 42 °C, and at pH 10·7. Strain 042B was shown to have two large plasmids (molecular sizes 200 kb and > 1000 kb, respectively). The DNA G + C mol% of strain 042B was 63·6. The DNA level of homology between strain 042B and type-strain USDA1002 was 76%, while DNA homologies of strain 042B were 10% and 12% with USDA205 and USDA6, respectively.

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1995-08-01
2021-10-16
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References

  1. Button J.C. 1980; New developments in inoculating legumes. In Recent Advances in Biological Nitrogen Fixation pp. 380–405 Subba Rao N.S. Edited by London:: Arnold Press.;
    [Google Scholar]
  2. Chen W.X., Li G.S., Qi Y.L., Wang E.T., Yuan H.L., Li J.L. 1991; Rhiɀobium huakuii sp. nov. isolated from the root nodules of Astragalus sinicus. Int J Syst Bacteriol 41:275–280
    [Google Scholar]
  3. Chen W.X., Wu B.H., Luo C.H., Li J.L., Shi Y.H., Huang L., Wu Z.Y. 1987; The survey and taxonomy of leguminous root nodule bacteria of Xinjiang region. Sci Agric Sin 20:22–27 (in Chinese)
    [Google Scholar]
  4. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101:738–754
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Fur J Biochem 12:133–142
    [Google Scholar]
  6. Denarie J., Debelle F., Truchet G., Promé J.-C. 1993; Rhizobium and legume nodulation: a molecular dialogue. In New Horizons in Nitrogen Fixation pp. 19–30 Palacios R. et al. The Netherlands:: Kluwer Academic Publishers.;
    [Google Scholar]
  7. Eardly B.D., Materon L.A., Smith N.H., Johnson D.A., Rumbaugh M.D., Selander R.K. 1990; Genetic structure of natural populations of the nitrogen-fixing bacterium Rhiɀobium meliloti. Appl Environ Microbiol 56:187–194
    [Google Scholar]
  8. Eardly B.D., Young J.P.W., Selander R.K. 1992; Phylogenetic position of Rhiɀobium sp. strain Or 191, a symbiont of both Medicago sativa and Phaseolus vulgaris, based on partial sequences of the 16S rRNA and nifH genes. Appl Environ Microbiol 58:1809–1815
    [Google Scholar]
  9. Eckhardt T. 1978; A rapid method for the identification of plasmid DNA in bacteria. Plasmid 1:584–588
    [Google Scholar]
  10. Fuhrmann J., Wollum A.G. II 1985; Simplified enzyme-linked immunosorbent assays for routine identification of Rhiɀobium japonicum antigens. Appl Environ Microbiol 49:1010–1013
    [Google Scholar]
  11. Hardy R.W.F., Burns R.C., Holsten R.D. 1973; Applications of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biol Biochem 5:47–81
    [Google Scholar]
  12. Jarvis B.D.W., Downer H.L., Young J.P.W. 1992; Phylogeny of fast-growing soybean-nodulating rhizobia supports synonymy of Sinorhiɀobium and Rhiɀobium and assignment to Rhiɀobium fredii. Ini J Syst Bacteriol 42:93–96
    [Google Scholar]
  13. Johnson J.L. 1985; Determination of DNA base composition, DNA reassociation and DNA hybridization of bacterial nucleic acid. Methods Microbiol 18:1–74
    [Google Scholar]
  14. Jordan D.C. 1984; Family III. Rhizobiaceae Conn 1938,321 AL . In Bergey̓s Manual of Systematic Bacteriology I pp. 234–242 Krieg N.R., Holt J.G. Edited by Baltimore:: Williams & Wilkins.;
    [Google Scholar]
  15. Marmur J., Doty P. 1962; Determination of the base composition of DNA from its thermal denaturation temperature. J Mol Biol 5:109–118
    [Google Scholar]
  16. Sadowsky M.J., Bohlool B.B., Keyser H.H. 1987; Serological relatedness of Rhiɀobium fredii to other rhizobia and to the bradyrhizobia. Appl Environ Microbiol 5:1785–1789
    [Google Scholar]
  17. Schmidt E.L., Bankole R.O., Bohlool B.B. 1968; Fluorescent antibody approach to the study of rhizobia in soil. J Bacteriol 95:1987–1992
    [Google Scholar]
  18. Vincent J.M. 1970 A Manual for the Practical Study of the Root-Nodule Bacteria Oxford:: Blackwell Scientific Publications.;
    [Google Scholar]
  19. Vincent J.M. 1982 Nitrogen Fixation in Legumes Sydney:: Academic Press.;
    [Google Scholar]
  20. Yang S.S., Li J.L. 1988; Salt tolerance and nodulation characteristics of fast-growing soybean rhizobia. Acta Agric Univ Pek 14:143–148 (in Chinese)
    [Google Scholar]
  21. Yu Y., Xu L.M., Fan H., Ge C. 1992; A study on the plasmid profiles and plasmid deletion of Sinorhiɀobium fredii strains from different origins. Sci Agric Sin 25:14–21 (in Chinese)
    [Google Scholar]
  22. Zhou P.J., Den J.L., Zhang X.J., Wu S.T. 1979; Nodulation experiments with legume using a hydroponic method. Oil Crops China 2:60–62 (in Chinese)
    [Google Scholar]
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