Summary: The SpollAB protein of is an anti-sigma factor that controls the release of - in the prespore during sporulation. A missense mutation, , in the N-terminal coding portion of was isolated previously on the basis of conferring increased - activity on agar plates. We present the results of experiments further characterizing the phenotypic effects of the mutation. The mutation severely impairs spore formation on nutrient agar (NA) plates. Surprisingly, however, cultures induced to sporulate in liquid medium by the resuspension method showed little or no reduction in spore formation, and gene expression was more or less normal. The effects on sporulation on NA can be suppressed by mutations in the gene encoding -. The implications for our understanding of the regulation of - and - activities, and the nature of the apparent medium dependence of sporulation in the mutant, are discussed.


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