1887

Abstract

SUMMARY

Specific pathogen-free C57BL/6 mice infected with recombinant (r) bearing BCG genes showed increased splenic survival compared to those receiving the vector control (plasmid DNA only). The mouse-passaged r (J3R) survived in peritoneal macrophages better than the vector control, regardless of whether the macrophages were infected . When r J3R was cultured in synthetic Proskauer-Beck-Tween medium, protein bands characteristic of BCG culture filtrates and not present in the vector control preparation were observed. Mice immunized with two doses of heat-killed J3R suspended in Freund's adjuvant were able to limit the growth of virulent within the lung and spleen compared to that observed in control mice receiving adjuvanted vector control or Freund's adjuvant alone.

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1995-05-01
2021-04-19
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References

  1. Bloom B.R. 1989; New approaches to vaccine development.. Rev Infect Dis 11:S460–466
    [Google Scholar]
  2. Collins F.M. 1972; Salmonellosis in orally infected specific pathogen-free C57BL/6 mice.. Infect Immun 5:191–198
    [Google Scholar]
  3. Collins F.M. 1983; Kinetics of the delayed-type hypersensitivity response in tuberculous guinea pigs and mice tested with several mycobacterial antigen preparations.. Am Rev Respir Dis 127:599–604
    [Google Scholar]
  4. Collins F.M. 1984; Protection against mycobacterial disease by means of live vaccines tested in experimental animals.. In The Mycobacteria: a Casebook 2 pp. 787–839 Edited by Kubica G. P., Wayne L. G. New York: Marcel Dekker;
    [Google Scholar]
  5. Collins F.M. 1985; Protection afforded by BCG vaccines against an aerogenic challenge by three mycobacteria of decreasing virulence.. Tubercle 66:267–276
    [Google Scholar]
  6. Collins F.M. 1991; Anti-tuberculous immunity: new solutions to an old problem.. Rev Infect Dis 13:940–950
    [Google Scholar]
  7. Collins F.M., Mackaness G.B. 1970a; The relationship of delayed hypersensitivity to acquired antituberculous resistance. I. Tuberculin sensitivity and resistance to reinfection in BCG-vaccinated mice.. Cell Immunol 1:253–265
    [Google Scholar]
  8. Collins F.M., Mackaness G.B. 1970b; The relationship of delayed hypersensitivity to acquired antituberculous resistance. II. Effect of adjuvant on the allergenicity and immunogenicity of heat-killed tubercle bacilli.. Cell Immunol 1:266–275
    [Google Scholar]
  9. Collins F.M., Lamb J.R., Young D.B. 1988; Biological activity of protein antigens isolated from Mycobacterium tuberculosis culture filtrate.. Infect Immun 56:1260–1266
    [Google Scholar]
  10. Curtiss R., Kelly S.M., Gulig P.A., Nahagama K. 1989; Selective delivery of antigens by recombinant bacteria.. Curr Top Microbiol 146:35–49
    [Google Scholar]
  11. Denis M., Forget A., Pelletier M., Servais F., Skamene E. 1990; Killing of Mycobacterium smegmatis by macrophages from genetically susceptible and resistant mice.. J Leukocyte Biol 47:25–30
    [Google Scholar]
  12. Fine P.E.M. 1989; The BCG story: lessons from the past and implications for the future.. Rev Infect Dis 11:S353–359
    [Google Scholar]
  13. Grosskinsky C.M., Jacobs , Clark-Curtiss J., Bloom B.R. 1989; Genetic relationships among Mycobacterium leprae, M. tuberculosis and candidate leprosy vaccine strains determined by DNA hybridization: identification of an M. leprae-specific repetitive sequence.. Infect Immun 57:1535–1541
    [Google Scholar]
  14. Hubbard R.D., Collins F.M. 1991; Immunomodulation of mouse macrophage killing of Mycobacterium avium in vitro.. Infect Immun 59:570–574
    [Google Scholar]
  15. Hubbard R.D., Flory C.M., Collins F.M. 1992; Immunization of mice with mycobacterial culture filtrate proteins.. Clin Exp Immunol 87:94–98
    [Google Scholar]
  16. Jacobs W.R., Kalpana G.V., Cirillo J.D., Pascopella L., Snapper S.B., Udani R.A., Jones W., Barletta R.G., Bloom B.R. 1991; Genetic systems for mycobacteria.. Methods Ensymol 204:537–555
    [Google Scholar]
  17. Kim T.H., Kubica G.P. 1972; Long term preservation and storage of mycobacteria.. Appl Microbiol 24:311–317
    [Google Scholar]
  18. Lagrange P.H., Miller T.E., Mackaness G.B. 1976; Parameters conditioning the potentiating effect of BCG on the immune response.. In BCG in Cancer Immunotherapy pp. 23–36 Edited by Lamoureux G., Turcotte R., Portelance V. New York: Grune and Stratton;
    [Google Scholar]
  19. Orme I.M. 1988; Characteristics and specificity of acquired immunologic memory to Mycobacterium tuberculosis infection.. J Immunol 140:3589–3593
    [Google Scholar]
  20. Pascopella L, Collins F. M., Martin J.M., Lee M.H., Hatfull G.F., Stover C.K., Bloom B.R., Jacobs W.R. 1994; Use of in vivo complementation in Mycobacterium tuberculosis to identify a genomic fragment associated with virulence.. Infect Immun 62:1313–1319
    [Google Scholar]
  21. Snapper S.B., Lugosi L., Jekkel A., Melton R.E., Kieser T., Bloom B.R., Jacobs W.R. 1988; Lysogeny and transformation of mycobacteria: stable expression of foreign genes.. Proc Natl Acad Sci USA 856987–6991
    [Google Scholar]
  22. Snapper S.B., Melton R.E., Mustafa S., Kieser T., Jacobs W.R. 1990; Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis.. Mol Microbiol 4:1911–1919
    [Google Scholar]
  23. Stokes R.W., Collins F.M. 1988; Growth of Mycobacterium avium in activated macrophages harvested from inbred mice of different innate susceptibilities to mycobacterial infection.. Infect Immun 56:2250–2254
    [Google Scholar]
  24. Stokes R.W., Orme I.M., Collins F.M. 1986; The role of phagocytes in the expression of resistance and susceptibility to Mycobacterium avium in mice.. Infect Immun 54:811–819
    [Google Scholar]
  25. Sudre P., ten Dam G., Kochi A. 1992; Tuberculosis: a global overview of the situation today.. Bull WHO 70:149–159
    [Google Scholar]
  26. Wiegeshaus E.H., Harding G., McMurray D., Grover A.A., Smith D.W. 1971; A co-operative evaluation of test systems used to assay tuberculosis vaccines.. Bull WHO 45:543–550
    [Google Scholar]
  27. World Health Organization 1993; The global tuberculosis emergency.. Press Release WHO/31
    [Google Scholar]
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