Specific pathogen-free C57BL/6 mice infected with recombinant (r) bearing BCG genes showed increased splenic survival compared to those receiving the vector control (plasmid DNA only). The mouse-passaged r (J3R) survived in peritoneal macrophages better than the vector control, regardless of whether the macrophages were infected . When r J3R was cultured in synthetic Proskauer-Beck-Tween medium, protein bands characteristic of BCG culture filtrates and not present in the vector control preparation were observed. Mice immunized with two doses of heat-killed J3R suspended in Freund's adjuvant were able to limit the growth of virulent within the lung and spleen compared to that observed in control mice receiving adjuvanted vector control or Freund's adjuvant alone.


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