1887

Abstract

SUMMARY

A peptide transport gene, , was cloned from a genomic library by functional complementation of a peptide transport deficient mutant (strain 2-2) of restored peptide transport to transformants as determined by uptake of radiolabelled dileucine, growth on dipeptides as sources of required amino acids, and restoration of growth inhibition by toxic peptides. Plasmid curing experiments demonstrated that the peptide transport phenotype was plasmid borne. was localized to chromosome R of by contour-clamped homologous electric field gel chromosome blots. Deletion subclones and frameshift mutagenesis were used to narrow the peptide transport complementing region to a 5:1 kb DNA fragment. DNA sequencing of the complementing region identified an ORF of 1869 bp containing an 84 nucleotide intron. The deduced amino acid sequence predicts a protein of 70 kDa consisting of 623 amino acids with 12 hydrophobic segments. A high level of identity was found between the predicted protein and peptide transport proteins of and This study represents the first steps in the genetic characterization of peptide transport in and initiates a molecular approach for the study of drug delivery against this pathogen.

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/content/journal/micro/10.1099/13500872-141-5-1147
1995-05-01
2019-10-15
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