1887

Abstract

SUMMERY:

The ability of to bind to fibrinogen and fibrin is believed to be an important factor in the initiation of foreign body and wound infections. Recently, the gene encoding the fibrinogen receptor (clumping factor, ClfA) of strain Newman was cloned and sequenced. The ClfA protein possesses a highly unusual 308 residue dipeptide repeat region composed predominantly of Asp and Ser. Polymerase chain reaction analysis of seven different strains showed that the size of the repeat coding region varies from 580 bp to 1320 bp. In contrast, the region A is the same size in each strain. The size of the repeat region did not correlate with the ability of these strains to form clumps in a solution of fibrinogen. Indeed, the strain with the smallest repeat size of 580 bp clumped almost as well as strain Newman. Each strain of examined contained several high molecular mass proteins that reacted with anti-ClfA region A antibody. In some cases the molecular mass of the major protein varied in accordance with the length of the coding sequence for the repeat region R.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-4-937
1995-04-01
2021-07-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/4/mic-141-4-937.html?itemId=/content/journal/micro/10.1099/13500872-141-4-937&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Smith J. A., Seidman J. G., Struhl K. 1987 Current Protocols in Molecular Biology New York: John Wiley;
    [Google Scholar]
  2. Bollet C., Gevaudan M. J., deLamballerie X., Zandotti C., deMicco P. 1991; A simple method for the isolation of chromosomal DNA from Gram positive or acid fast bacteria. Nucleic Acids Res 19:1955
    [Google Scholar]
  3. Cheung A., Fischetti V. A. 1989; Role of surface proteins in staphylococcal adherence to fibres in vitro.. J Clin Invest 83:2041–2049
    [Google Scholar]
  4. Cottonaro C. N., Roohk H. V., Shimica G., Sperling D. R. 1981; Quantitation and characterisation of competitive protein binding to polymers. Trans Am Soc Artif Intern Organs 27:391–395
    [Google Scholar]
  5. Duthie E. S., Lorenz L. L. 1952; Staphylocoagulase: mode of action and antigenicity. J Gen Microbiol 6:95–107
    [Google Scholar]
  6. Fischetti V. A., Jarymowycz M., Jones K. F., Scott J. R. 1986; Streptococcal M protein size mutants occur at high frequency within a single strain. J Exp Med 164:971–980
    [Google Scholar]
  7. Guss B., Uhlen M., Nilsson B., Lindberg M., Sjoquist J., sjodahl J. 1984; Region X, the cell-wall-attachment part of staphylococcal protein A. Eur J Biochem 138:413–420
    [Google Scholar]
  8. Hawiger J. S., Timmons S., Strong D. D., Cottrell B. A., Riley M., Doolittle R. F. 1982; Identification of a region of human fibrinogen interacting with staphylococcal clumping factor. Bio-chemistry 21:1407–1413
    [Google Scholar]
  9. Hollingshead S. K., Fischetti V. A., Scott J. R. 1987; Size variation in group A streptococcal M protein is generated by homologous recombination between intragenic repeats. Mol & Gen Genet 207:196–203
    [Google Scholar]
  10. Humphreys H., Keane C. T., Hone R., Pomeroy H., Russel R. J., Arbuthnott J. P., Coleman D. C. 1989; Enterotoxin production by Staphylococcus aureus isolates from cases of septicaemia and from healthy carriers. J Med Microbiol 28:163–172
    [Google Scholar]
  11. Jones T. O., Wiencke A. A. 1986; Staphylococcal toxic shock syndrome. Vet Res 25:435–436
    [Google Scholar]
  12. Jonsson K., Signas C., Muller H.-P, Lindberg M. 1992; Two different genes encode fibronectin binding proteins in Staphylococcus aureus. The complete nucleotide sequence and characterisation of the second gene. Eur J Biochem 356:1–8
    [Google Scholar]
  13. Kristinsson K. G. 1980; Adherence of Staphylococci to intravascular catheters. J Med Microbiol 28:249–257
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  15. Maki D. G. 1982 Infections associated with intravascular lines. In Current Topics in Infectious Diseases 3309–363 Edited by Remington J. S., Swartz N. M. New York: McGraw Hill;
    [Google Scholar]
  16. McDevitt D., Vaudaux P., Foster T. J. 1992; Genetic evidence that bound coagulase of Staphylococcus aureus is not the clumping factor. Infect Immun 60:1514–1523
    [Google Scholar]
  17. McDevitt D., Francois P., Vaudaux P., Foster T. J. 1994; Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus.. Mol Microbiol 11:237–248
    [Google Scholar]
  18. Olsson A., Eliasson M., Guss B., Nilsson B., Heilman U., Lind berg M., Uhlen M. 1987; Structure and evolution of the repetitive gene encoding streptococcal protein G. Eur J Biochem 168:319–324
    [Google Scholar]
  19. O’Reilly M., Kreiswirth B., Foster T. J. 1990; Cryptic α-toxin gene in toxic shock syndrome and septicaemia strains of Staphylococcus aureus.. Mol Microbiol 4:1947–1955
    [Google Scholar]
  20. Phonimdaeng P., O’Reilly M., Nowlan P., Bramley A. J., Foster T. J. 1990; The coagulase of Staphylococcus aureus. Sequence analysis and virulence of site specific coagulase deficient mutants. Mol Microbiol 4:393–404
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Shanson D. C. 1990; Staphylococcal infections in hospitals. Br J Hosp Med55–312
    [Google Scholar]
  23. Signas C., Raucci G., Jonsson K., Lindgren P. E., Anan- tharamaiah G. M., Hook M., Lindberg M. 1989; Nucleotide sequence of the gene for a fibronectin-binding protein from Staphylococcus aureus: use of this peptide sequence in the synthesis of biologically active peptides. Proc Natl Acad Sci USA 86:699–703
    [Google Scholar]
  24. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
    [Google Scholar]
  25. Stern A., Brown M., Nickel P., Meyer T. F. 1986; Opacity genes in Neisseria gonorrhoeae : control of phase and antigenic variation. Cell 47:61–67
    [Google Scholar]
  26. Uhlen M., Guss B., Nilsson B., Gatenbeck S., Philipson L., Lindberg M. 1984; Complete sequence of the staphylococcal gene encoding protein A, a gene evolved through multiple duplications. J Biol Chem 259:1695–1702
    [Google Scholar]
  27. Vaudaux P., Pittet D., Haeberli A., Huggler E., Kydegger U. E., Lew D. P., Waldvogel F. A. 1989; Host factors selectively increase staphylococcal adherence on inserted catheters. A role for fibronectin and fibrinogen or fibrin. J Infect Dis 160:865–875
    [Google Scholar]
  28. Vaudaux P. E., Francois P., Proctor R. A., McDevitt D., Foster T. J., Albrecht R. M., Lew D. P., Wabers H., Cooper S. L. 1995; Use of adhesion-defective mutants of Staphylococcus aureus to define the role of specific plasma proteins in promoting bacterial adhesion to canine arterio-venous shunts. Infect Immun 63 (in press)
    [Google Scholar]
  29. Vaudaux P., Pittet D., Haeberli A., Lerch P. G., Morgenthaler J. J., Proctor R. A., Waldvogel F. A., Lew D. P. 1993; Fibronectin is more active than fibrin or fibrinogen in promoting Staphylococcus aureus adherence to inserted intravascular catheters. J Infect Dis 167:633–641
    [Google Scholar]
  30. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-4-937
Loading
/content/journal/micro/10.1099/13500872-141-4-937
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error