1887

Abstract

SUMMARY

The location and organization of repetitive sequences, members of the RPS family, which are sequences specific to , were determined on each chromosome of strain FC18. Using pulsed-field gel electrophoresis, we separated seven fractions from eight chromosomes. Each chromosome was cleaved by HI and oI to excise the RPSs, which were then detected by hybridization with an RPS probe. All chromosomes except chromosome 4 carried RPSs, and these RPSs were located within a limited region on each chromosome. From the digestion of each chromosome with l and probing with the RPSs, we found that these recognition sites within the RPS region were conserved among all RPS-containing chromosomes. For further characterization of the RPSs, the locations and the boundary regions of the RPSs were examined on chromosome 6 of strain FC18 as a model chromosome. Using the restriction enzymes l, l, ol, Hl, ul and l, we constructed a semi-macro physical map of the RPSs and their boundary regions on this chromosome. We also determined which part of the RPS was adjacent to each boundary by using sub-fragments of RPS as probes. The physical configuration around the RPSs and their boundary regions are presented. The results obtained should be useful for future analysis of the function of these regions.

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1995-02-01
2021-10-20
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References

  1. Anderson J., Srikantha T., Morrow B., Miyasaki S. H., White T. C., Agabian N., Schmid J., Soli D. R. 1993; Characterization and partial nucleotide sequence of the DNA fingerprinting probe Ca3 of Candida albicans . J Clin Microbiol 31:1472–1480
    [Google Scholar]
  2. Barton R. C., Scherer S. 1994; Induced chromosome rearrangements and morphologic variation in Candida albicans . J Bacterial 176: 756–763
    [Google Scholar]
  3. Centola M., Carbon J. 1994; Cloning and characterization of centromeric DNA from Neurospora crassa . Mol Cell Biol 14 : 1510–1519
    [Google Scholar]
  4. Chibana H., Iwaguchi S.-I., Homma M., Chindamporn A., Nakagawa Y., Tanaka K. 1994; Diversity of tandemly repetitive sequences due to periodic repetitions in the chromosomes of Candida albicans . J Bacteriol 176:3851–3858
    [Google Scholar]
  5. Chindamporn A., Iwaguchi S.-I., Nakagawa Y., Homma M., Tanaka K. 1993; Clonal size-variation of rDNA cluster region on chromosome XII of Saccharomyces cerevisiae . J Gen Microbiol 139: 1409–1415
    [Google Scholar]
  6. Chu W.-S., Magee B. B., Magee P. T. 1993; Construction of an Sfil macrorestriction map of the Candida albicans genome. . J Bacteriol 175: 6637–6651
    [Google Scholar]
  7. Clarke L. 1990; Centromeres of budding and fission yeasts. Trends Genet 6: 150–154
    [Google Scholar]
  8. Doi M., Homma M., Chindamporn A., Tanaka K. 1992; Estimation of chromosome number and size by pulsed-field gel electrophoresis (PFGE) in medically important Candida species. J Gen Microbiol 138: 2243–2251
    [Google Scholar]
  9. Iwaguchi S.-I., Homma M., Tanaka K. 1990; Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans . J Gen Microbiol 136: 2433–2442
    [Google Scholar]
  10. Iwaguchi S.-I., Homma M., Chibana H., Tanaka K. (1992a); Isolation and characterization of a repeated sequence (RPS1) of Candida albicans . J Gen Microbiol 138: 1893–1900
    [Google Scholar]
  11. Iwaguchi S.-I., Homma M., Tanaka K. 1992b; Clonal variation of chromosome size derived from the rDNA cluster region in Candida albicans . J Gen Microbiol 138: 1177–1184
    [Google Scholar]
  12. Kurtz M. B., Cortelyou M. W., Miller S. M., Kirsch D. R. 1987; Development of autonomously replicating plasmids for Candida albicans . Mol Cell Biol 7: 209–217
    [Google Scholar]
  13. Lasker B. A., Carle G. F., Kobayashi G. S., Medoff G. 1989; Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques. Nucleic Acids Res 17: 3783–3793
    [Google Scholar]
  14. Lasker B. A., Page L. S., Lott T. J., Kobayashi G. S., Medoff G. 1991; Characterization of CARE-1: Candida albicans repetitive element-1. Gene 102: 45–50
    [Google Scholar]
  15. Lott T. J., Boiron P., Reiss E. 1987; An electrophoretic karyotype for Candida albicans reveals large chromosomes in multiples. Mol & Gen Genet 209: 170–174
    [Google Scholar]
  16. Magee B. B., Magee P. T. 1987; Electrophoretic karyotypes and chromosome numbers in Candida species. J Gen Microbiol 133:425–430
    [Google Scholar]
  17. Magee B. B., Koltin Y., Gorman J. A., Magee P. T. 1988; Assignment of cloned genes to the seven electrophoretically separated Candida albicans chromosomes. Mol Cell Biol 8:4721–4726
    [Google Scholar]
  18. McEachern M. J., Hicks J. B. 1993; Unusually large telomeric repeat in the yeast Candida albicans . Mol Cell Biol 13: 551–560
    [Google Scholar]
  19. Rustchenko-Bulgac E. P., Sherman F., Hicks J. B. 1990; Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans . J Bacteriol 172:1276–1283
    [Google Scholar]
  20. Sadhu C., McEachern M. J., Rustchenko-Bulgac E. P., Schmid J., Soll D. R., Hicks J. B. 1991; Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification. J Bacteriol 173:842–850
    [Google Scholar]
  21. Scherer S., Stevens D. A. 1988; A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl AcadSci USA 85:1452–1456
    [Google Scholar]
  22. Schwartz D. C., Cantor C. R. 1984; Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75
    [Google Scholar]
  23. Suzuki T., Kobayashi I., Kanbe T., Tanaka K. 1989; High- frequency variation of colony morphology and chromosome reorganization in the pathogenic yeast Candida albicans . J Gen Microbiol 135:425–434
    [Google Scholar]
  24. Takahashi K., Murakami S., Chikashige Y., Funabiki H., Niwa O., Yanagida M. 1992; A low copy number central sequence with strict symmetry and unusual chromatin structure in fission yeast centromere. Mol Biol Cell 3:819–835
    [Google Scholar]
  25. Thrash-Bingham C., Gorman J. A. 1993; Identification, characterization and sequence of Candida albicans repetitive DNAs Rel-1 and Rel-2. Curr Genet 23:455–462
    [Google Scholar]
  26. Vogt P. 1992; Code domains in tandem repetitive DNA sequence structures. Chromosoma 101:585–589
    [Google Scholar]
  27. Willard H. F., Waye J. S. 1987; Hierarchical order in chromosome-specific human alpha satellite DNA. Trends Genet 3:192–198
    [Google Scholar]
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