1887

Abstract

Adaptation of to short-term stress induced by three antifungal agents [amphotericin B (AMPH), miconazole (MCZ), and ketoconazole (KCZ)] was observed and evaluated quantitatively using individual hyphae. Spores were inoculated onto a poly--lysine-coated glass plate making up the base of a culture vessel. Potato dextrose broth (PDB) was added and the vessel incubated for 24 h at 28 °C. The growth rate of an arbitrarily selected test hypha was measured automatically. Exposure to AMPH (0.075 μg ml) stopped the growth of the hypha. After washing with PDB, the same concentration of AMPH was applied again. The growth of the test hypha was not inhibited. This phenomenon was defined as adaptation to the short-term stress of AMPH. Similarly, adaptation was observed with MCZ (0.01 μg ml) and KCZ (0.5 μg ml). The time required for the test hypha to restart growth after washing with PDB depended upon the concentration of MCZ or KCZ, but not upon the concentration of AMPH.

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1994-09-01
2021-10-25
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References

  1. Bolard J. 1986; How do the polyene macrolide antibiotics affect the cellular membrane properties?. Biochim Biophjs Acta 864:257–304
    [Google Scholar]
  2. Collison L.P., Dawes I. W. 1992; Inducibility of the response of yeast cells to peroxide stress. J Gen Microbiol 138:329–335
    [Google Scholar]
  3. Hiratani T., Yamaguchi T. 1985; Mode of antifungal action of oxiconazole nitrate toward Candida albicans.. Chemotherapy 33:215–226
    [Google Scholar]
  4. Lenhart K. 1973; Population aspects in genetics of dermatophytes. Acta Univ Valacki Olomuc Fac Med 65:59–74
    [Google Scholar]
  5. Lenhart K., Merkunova A. 1989; Adaptation of Microsporum gypseum to ketoconazole.. Acta Univ Palacki Olomuc Fac Med 123:35–47
    [Google Scholar]
  6. Oh K., Matsuoka H., Sumita O., Takatori K., Kurata H. 1992; Evaluation of antifungal activity of antimycotics by automatic analyzing system. Mycopathologia 118:71–81
    [Google Scholar]
  7. Oh K., Matsuoka H., Sumita O., Takatori K., Kurata H. 1993a; Automatic evaluation of antifungal volatile compounds on the basis of the dynamic growth process of a single hypha. Appl Microbiol Biotechnol 38:790–794
    [Google Scholar]
  8. Oh K., Matsuoka H., Nemoto Y., Sumita O., Takatori K., Kurata H. 1993b; Determination of anti-Aspergillus activity of antifungal agents based on the dynamic growth rate of a single hypha.. Appl Microbiol Biotechnol 39:363–367
    [Google Scholar]
  9. Park J.-C., Nemoto Y., Homma T., Jing W., Chen Y., Matsuoka H., Ohno H., Takatori K., Kurata H. 1993; Adaptation of Aspergillus niger to short-term salt stress.. Appl Microbiol Biotechnol 40:394–398
    [Google Scholar]
  10. Ryley J. F., Wilson R. G., Barrett-Bee K.J. 1984; Azole resistance in Candida albicans.. Sabouraudia 22:53–63
    [Google Scholar]
  11. Schroeder L.L., Bullerman L. B. 1985; Potential for development of tolerance by Penicillium digitatum and Penicillium italicum after repeated exposure to potassium sorbate.. Appl Environ Microbiol 50:919–923
    [Google Scholar]
  12. Van den Bossche H., Willemsens G., Cools W., Lauwers W. F. J., Le Jeune L. 1978; Biochemical effects of miconazole on fungi. II. Inhibition of ergosterol biosynthesis in Candida albicans.. Chem Biol Interact 21:59–78
    [Google Scholar]
  13. Yamada S., Cao J., Sumita O., Kurasawa K., Kurata H., Oh K., Matsuoka H. 1992; Automatic antifungal activity analyzing system on the basis of dynamic growth process of a single hypha. Mycopathologia 118:65–69
    [Google Scholar]
  14. Yamaguchi H., Hiratani T. 1984; Studies on the mode of antifungal action of bifonazole. Chemotherapy 32:829–841
    [Google Scholar]
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