1887

Abstract

The organization of the components of the cell wall from was studied by means of sequential treatment with hot SDS, anhydrous ethylenediamine (EDA) and lytic enzymes, followed by chemical and microscopic analyses of the different separated fractions. The EDA-insoluble fraction retained the original morphology of the wall, which was destroyed by β-glucanase, but not by chitinase treatments. Staining with fluorescent lectins revealed distinct distributions of mannoproteins, glucans and chitin in the wall. Amino acid analysis of SDS-extracted walls, and the EDA-soluble and -resistant fractions gave similar results, with seven amino acids making up about 70% of the total protein weight. Treatment of the EDA-insoluble fraction with Zymolyase or chitinase released fragments of variable size whose susceptibility to these and other hydrolases suggests that they are made of glucan, chitin and mannan oligomers associated with proteins. Treatment of the Zymolyase-insoluble residue with chitinase released a series of low-molecular-mass oligomers made of neutral sugars, GIcNAc and amino acids, mainly lysine. It is suggested that they represent fragments of the core making up the scaffold of the cell wall of the fungus.

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1994-07-01
2021-05-09
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References

  1. Benitez T., Villa T.G., Garcia-Acha I. Some chemical and structural features of the conidial wall of Trichoderma viride. Can J Microbiol 1976; 22:3318–3321
    [Google Scholar]
  2. Braun P.C., Calderone R.A. Chitin synthesis in Candida albicans-, comparison of yeast and hyphal forms. J Bacterial 1978; 133:1472–1477
    [Google Scholar]
  3. Chaffin W.L., Stocco D.M. Cell wall mannoproteins of Candida albicans. Can J Microbiol 1983; 29:1438–1444
    [Google Scholar]
  4. Chattaway F.W., Holmes M.R., Barlow A.J.E. Cell wall composition of the mycelial and blastospore forms of Candida albicans. J Gen Microbiol 1968; 51:367–376
    [Google Scholar]
  5. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956; 28:350–356
    [Google Scholar]
  6. Elorza M.V., Rico H., Sentandreu R. Calcofluor white alters the assembly of chitin microfibrils in Saccharomyces cerevisiae. J Gen Microbiol 1983; 128:1577–1582
    [Google Scholar]
  7. Elorza M.V., Murgui A., Sentandreu R. Dimorphism in Candida albicans-, contribution of mannoproteins to the architecture of yeast and mycelial cell walls. J Gen Microbiol 1985; 131:2209–2216
    [Google Scholar]
  8. Elorza M.V., Marcilla A., Sentandreu R. Wall mannoproteins of the yeast and mycelial cells of Candida albicans: nature of the glycosidic bonds and polydispersity of their mannan moieties. J Gen Microbiol 1988; 134:2393–2403
    [Google Scholar]
  9. Elorza M.V., Mormeneo S., Garcia De La Cruz R.F., Sentandreu R. Evidence for the formation of covalent bonds between macromolecules in the domain of the wall of Candida albicans mycelial walls. Biochem Biophys Res Commun 1989; 162:1118–1125
    [Google Scholar]
  10. Frevert J., Ballou C.E. Saccharomyces cerevisiae structural cell wall mannoprotein. Biochemistry 1985; 24:753–759
    [Google Scholar]
  11. Herrero E., Sanz A., Sentandreu R. Cell wall proteins liberated by zymolyase from several ascomycetous and imperfect yeasts. J Gen Microbiol 1987; 133:2895–2903
    [Google Scholar]
  12. Herth H. Calcofluor white and congo red inhibit chitin microfibril assembly of Poterioochromonas. Evidence for a gap between polymerization and microfibril formation. T Cell Biol 1980; 87:442–450
    [Google Scholar]
  13. Horisberger M., Volanthen M. Localization of mannan and chitin on thin sections of budding yeast with gold markers. Arch Microbiol 1977; 115:1–7
    [Google Scholar]
  14. Korn E.D., Northcote D.H. Physical and chemical properties of polysaccharides and glycoproteins of the yeast cell wall. Biochem J 1960; 75:12–17
    [Google Scholar]
  15. Lee K., Buckley H.R., Campbell C.C. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans. Sabouraudia 1975; 13:148–153
    [Google Scholar]
  16. Lyon F.L., Domer J.E. Chemical and enzymatic variations in the cell wall of pathogenic Candida species. Can J Microbiol 1985; 31:590–597
    [Google Scholar]
  17. Marcilla A., Elorza M.V., Mormeneo S., Rico H., Sentandreu R. Candida albicans mycelial wall structure: supramolecular complexes released by Zymolyase, chitinase and β-mercapto-ethanol. Arch Microbiol 1991; 155:312–319
    [Google Scholar]
  18. Mol P.C., Wessels J.G.H. Linkages between glucosaminoglycan and glucan determine alkali-insolubility of the glucan in walls of Saccharomyces cerevisiae. FEMS Microbiol Lett 1987; 41:95–99
    [Google Scholar]
  19. Murgui A., Elorza M.V., Sentandreu R. Effect of papulacandin B and calcofluor white on the incorporation of mannoproteins in the wall of Candida albicans blastospores. Biochim Biophys Acta 1985; 841:215–222
    [Google Scholar]
  20. Pastor F.I.J., Valentin E., Herrero E., Sentandreu R. Structure of the Saccharomyces cerevisiae cell wall: mannoproteins released by zymolyase and their contribution to wall architecture. Biochim Biophys Acta 1984; 802:292–300
    [Google Scholar]
  21. Pollack J.H., Lange C.F., Hashimoto T. ‘Nonfibrillar’ chitin associated with walls and septa of Trichophyton mentagrophytes arthrospores. J Bacterial 1983; 154:965–975
    [Google Scholar]
  22. Reissig J.L., Strominger J.L., Leloir L.F. A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem 1955; 217:959–966
    [Google Scholar]
  23. Roberts W.K., Selitrennikoff C.P. Plant and bacterial chitinases differ in antifungal activity. J Gen Microbiol 1988; 134:169–176
    [Google Scholar]
  24. Ruiz-Herrera J. Fungal Cell Wall: Structure, Synthesis and Assembly 1992 Boca Raton: CRC Press;
    [Google Scholar]
  25. Sanz P., Herrero E., Sentandreu R. Autolytic release of mannoproteins from cell walls of Saccharomyces cerevisiae. J Gen Microbiol 1985; 131:2925–2932
    [Google Scholar]
  26. Sietsma J.H., Wessels J.G.H. Evidence for covalent linkages between chitin and β-glucan in a fungal wall. J Gen Microbiol 1979; 114:99–108
    [Google Scholar]
  27. Sietsma J.H., Wessels J.G.H. Solubility of (1 → 3)-β-D(1 → 6)- β-D-glucan in fungal walls: importance of presumed linkage between glucan and chitin. J Gen Microbiol 1981; 125:209–212
    [Google Scholar]
  28. Sonnenberg A.S.M., Sietsma J.H., Wessels J.G.H. Biosynthesis of alkali-insoluble cell-wall glucan in Schizophyllum commune protoplasts. J Gen Microbiol 1983; 128:2667–2674
    [Google Scholar]
  29. Surarit R., Gopal P.K., Shepherd M.G. Evidence for a glycosidic linkage between chitin and glucan in the wall of Candida albicans. J Gen Microbiol 1988; 134:1723–1730
    [Google Scholar]
  30. Trevelyan W.F., Procter D.P., Harrison J.S. Detection of sugars on paper chromatograms. Nature 1950; 166:444–445
    [Google Scholar]
  31. Valentin E., Herrero E., Pastor F.I.J., Sentandreu R. Solubilization and analysis of mannoprotein molecules from the cell wall of Saccharomyces cerevisiae. J Gen Microbiol 1984; 130:1419–1428
    [Google Scholar]
  32. Van Rinsum J., Klis F.M., Van Den Ende H. Cell wall glucomannoproteins of Saccharomjces cerevisiae mnn9. Yeast 1991; 7:717–726
    [Google Scholar]
  33. Vries O.M.H., Wessels J.G.H. Chemical analysis of cell wall regeneration and reversion of protoplasts from Schizophyllum commune. Arch Microbiol 1975; 102:209–218
    [Google Scholar]
  34. Wessels J.G.H., Sietsma J.H. Wall synthesis and assembly during hyphal morphogenesis in Schizophyllum commune. J Gen Microbiol 1983; 129:1607–1616
    [Google Scholar]
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