The alginate-like polysaccharides synthesized by and have been prepared from batch cultures grown with glucose and fructose as carbon substrates. Despite the different methods of catabolism of the two substrates and synthesis of the alginate precursors, both strains produced polysaccharides which were consistent in their composition of mannuronic and guluronic acids and in the frequency of occurrence of dimers of -mannuronic acid. All preparations lacked homooligomeric sequences of -guluronic acid and were highly acetylated (12–21%). In all the culture conditions tested, polysaccharide production was growth-associated and maximum was obtained after 48 h growth; older cultures contained material of progressively lower . This was ascribed to the degradative activity of alginate lyases which were detected intracellularly in both species and are presumably released by cell lysis. At 72 h, alginate from grown on either substrate had an of only 34000–38500, whereas the product from grown on fructose had an of 300000 and that from glucose-grown cultures an of 72000.


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