1887

Abstract

Summary: Disruption of the gene for the periplasmic cytochrome c in did not result in substantial alteration of rates of electron flow from physiological substrates to the reductases for nitrite, nitric oxide and nitrous oxide, thus confirming and extending earlier findings. In wild-type cells the chelating agent diethyldithiocarbamate (DDC) caused a partial inhibition of these electron transport processes. When the same concentrations of this inhibitor were added to a mutant of in which the gene for cytochrome c was specifically disrupted an almost complete inhibition of these reactions was observed. It is known from previous work with the closely related organism that DDC effectively and rapidly removes copper from the periplasmic pseudoazurin that is also found in . Therefore, it is concluded that in the absence of cytochrome c, a copper protein, probably pseudoazurin, acts as the electron carrier between the cytochrome complex and the reductases for nitrite, nitric oxide and nitrous oxide. Cells with the gene for cytochrome c deleted continued to give a positive Nadi test.

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1994-02-01
2021-10-18
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References

  1. Alefounder P. R., Ferguson S. J. 1980; The location of dissimilatory nitrite reductase and the control of dissimilatory nitrate reductase by oxygen in Paracoccus denitrificans. Biocbem J 192 231 240
    [Google Scholar]
  2. Alefounder P. R., Ferguson S. J. 1982; Electron transport-linked nitrous oxide synthesis and reduction by Paracoccus denitrificans monitored with an electrode. Biocbem Biophys Res Comm 104 1149 1155
    [Google Scholar]
  3. Alefounder P. R., McCarthy J. E. G., Ferguson S. J. 1981; The basis of the control of nitrate reduction by oxygen in Paracoccus denitrificans. FEMS Microbiol Lett 12 321 326
    [Google Scholar]
  4. Alefounder P. R., Greenfield A. J., McCarthy J. E. G., Ferguson S.J. 1983; Selection and organization of denitrifying electron transfer pathways in Paracoccus denitrificans. Biochim Biophys Acta 724 20 39
    [Google Scholar]
  5. Ambler R. P., Tobari J. 1985; The primary structures of Pseudomonas AMI amicyanin and pseudoazurin. Biocbem I 232 451 457
    [Google Scholar]
  6. Auton K. A., Anthony C. 1989a; The role of cytochromes and blue copper proteins in growth of an obligate methylotroph on methanol and methylamine. J Gen Microbiol 135 1923 1931
    [Google Scholar]
  7. Auton K. A., Anthony C. 1989b; The methylamine oxidase system of an obligate methylotroph. Biocbem J 260 75 79
    [Google Scholar]
  8. Bell L. C., Richardson D. J., Ferguson S. J. 1992; Identification of nitric oxide reductase activity in R hodobacter capsulatus: the electron transport pathway can either use or bypass both cytochrome c2 and the cytochrome bc1 complex. J Gen Microbiol 138 437 443
    [Google Scholar]
  9. Berks B. C., Baratta D., Richardson D. J., Ferguson S. J. 1993; Purification and characterization of a nitrous oxide reductase from Thiosphaera pantotropha Implications for the mechanism of aerobic nitrous oxide reduction. Eur J Biocbem 212 467 476
    [Google Scholar]
  10. Berry E. A., Trumpower B. L. 1985; Isolation of ubiquinol oxidase from Paracoccus denitrificans and resolution into cytochrome bc1 and cytochrome c aa.A complexes. J Biol Chem 260 2458 2467
    [Google Scholar]
  11. Boogerd F. C., van Verseveld H. W., Stouthamer A. H. 1980; Electron transport to nitrous oxide in Paracoccus denitrificans. FEBS Lett 113 279 284
    [Google Scholar]
  12. Boogerd F. C., , van Verseveld H. W., Stouthamer A. H. 1981; Respiratory-driven proton translocation with nitrite and nitrous oxide in Paracoccus denitrificans. Biochim Biophys Acta 638 181 191
    [Google Scholar]
  13. Bosma G., Braster M., Stouthamer A. H., van Verseveld H. W. 1987; Subfractionation and characterization of soluble r-type cytochromes from Paracoccus denitrificans cultured under various limiting conditions in the chemostat. Eur J Biocbem 165 665 670
    [Google Scholar]
  14. Bott M., Bolliger M., Hennecke H. 1990; Genetic analysis of the cytochrome c-aa3 branch of the Bradyrhfiobium japonicum respiratory chain. Mol Microbiol 4 2147 2157
    [Google Scholar]
  15. Carr G. J., Ferguson S. J. 1990; The nitric oxide reductase of Paracoccus denitrificans. Biocbem J 269 423 429
    [Google Scholar]
  16. Carr G. J., Page M. L. D., Ferguson S. J. 1989; The energy conserving nitric-oxide-reductase system in Paracoccus denitrificans. Eur J Biochem 179 683 692
    [Google Scholar]
  17. Coyne M. S., Arunakumari A., Averill B. A., Tiedje J. M. 1989; Immunological identification and distribution of dissimil-atory heme cd 1 and nonheme copper nitrite reductase in denitrifying bacteria. Appl Environ Microbiol 55 2924 2931
    [Google Scholar]
  18. Dermastia M., Turk T., Hollocher T. C. 1991; Nitric oxide reductase: purification from Paracoccus denitrificans with the use of a single column and some characteristics. J Biol Chem 266 10899 10905
    [Google Scholar]
  19. De Silva M. E. M. S. 1974; A method for the determination of copper in biological materials involving the use of sodium diethyldithiocarbamate. Analyst 99 408 412
    [Google Scholar]
  20. De Vries G. E., Harms N., Hoogendijk J., Stouthamer A. H. 1989; Isolation and charactrization of Paracoccus denitrificans mutants with increased conjugation frequencies and pleiotropic loss of a (nGATCn) DNA modifying property. Microbiol 152 52 57
    [Google Scholar]
  21. Ferguson S. J. 1987; Cytochrome c springs a surprise. Biochem Sci 12 124 125
    [Google Scholar]
  22. Goodhew C. F., Brown K. R., Pettigrew G. W. 1986; Haem staining in gels, a useful tool in the study of bacterial r-type cytochromes. Biophys Acta 852 288 294
    [Google Scholar]
  23. Harms N., De Vries G. E., Maurer K., Hoogendijk J., Stouthamer A. H. 1987; Isolation and nucleotide sequence of the methanol dehydrogenase structural gene from Paracoccus denitrificans. Bacteriol 169 3969 3975
    [Google Scholar]
  24. Ho K. K., Krogmann D. W. 1984; Electron donors to P700 in cyanobacteria and algae, an instance of unusual genetic variability. Biophys Acta 766 310 316
    [Google Scholar]
  25. Hoitink C. W. G., Woudt L. P., Turenhout J. C. M., Van De Kamp M., Canters G. W. 1990; Isolation and sequencing of the Alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis. 90 15 20
    [Google Scholar]
  26. Horio T., Higashi T., Yamanaka T., Matsubara H., Okunuki K. 1961; Purification and properties of cytochrome oxidase from Pseudomonas aeruginosa . Biol Chem 236 944 951
    [Google Scholar]
  27. Husain M., Davidson V. L. 1985; An inducible periplasmic blue copper protein from Paracoccus denitrificans. Biol Chem 260 14626 14629
    [Google Scholar]
  28. Jenney F. E., Daldal F. 1993; A novel membrane-associated c-type cytochrome, cyt c can mediate the photosynthetic growth of R hodobacter capsulatus and Khodobacter sphaeroides. EMBO J 12 1283 1292
    [Google Scholar]
  29. Jones M. R., McEwan A. G., Jackson J. B. 1990; The role of c-type cytochromes in the photosynthetic electron transport pathway of Khodobacter capsulatus. Biophys Acta 1019 59 66
    [Google Scholar]
  30. Jungst A., Wakabayashi S., Matsubara H., Zumft W. G. 1991; The nirSTBM region coding for cytochrome cd1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetrahaem proteins. Lett 279 205 209
    [Google Scholar]
  31. Kakutani T., Watanabe H., Arima K., Beppu T. 1981; A blue protein as an inactivating factor for nitrite reductase from Alcaligenes faecalis S-6. J Biochem 89 463 472
    [Google Scholar]
  32. Lam Y., Nicholas D. J. D. 1969; A nitrite reductase with cytochrome oxidase activity from Micrococcus denitrificans. et Biophys Acta 180 459 472
    [Google Scholar]
  33. Marrs B., Gest H. 1973; Genetic mutations affecting the respiratory electron transport system of the photosynthetic bacterium R hodopseudomonas capsulata. Bacteriol 114 1045 1051
    [Google Scholar]
  34. Martinkus K., Kennelly P. J., Rea T., Tinkovich R. 1980; Purification and properties of Paracoccus denitrificans azurin. Biochem Biophys 199 465 472
    [Google Scholar]
  35. Matchova I., Kucera I. 1991; Evidence for the role of soluble cytochrome c in the dissimilatory reduction of nitrite and nitrous oxide bv cells of Paracoccus denitrificans. Biophys Acta 1058 256 260
    [Google Scholar]
  36. Matchova I., Kucera I., Janiczek O., van Spanning R. M., Oltmann L. F. 1993; The existence of an alternative electron-transfer pathway to the periplasmic nitrite reductase (cytochrome cdf) in Paracoccus denitrificans. Microbiol 159 272 275
    [Google Scholar]
  37. McEwan A. G., Richardson D. J., Hudig H., Ferguson S. J., Jackson J. B. 1989; Identification of cytochromes involved in electron transport to trimethylamine N-oxide/dimethylsulphoxide reductase in Rhodobacter capsulatus. Biophys Acta 973 308 314
    [Google Scholar]
  38. Moir J. W. B., Baratta D., Richardson D. J., Ferguson S. J. 1993; The purification of a c-type nitrite reductase and the absence of a copper type nitrite reductase from the aerobic denitrifier Thiosphaera pantotropha ; the role of pseudoazurin as an electron donor. J Biochem 212 377 385
    [Google Scholar]
  39. Newton N. 1969; The two-haem nitrite reductase of Micrococcus denitrificans. Biochim Biophys Acta 185 316 331
    [Google Scholar]
  40. Page M. D., Carr G., Bell L. C., Ferguson S. J. 1989; Structure, control and assembly of a bacterial electron transport system as exemplified by Paracoccus denitrificans. Soc Trans 17 991 993
    [Google Scholar]
  41. Parr S. R., Barber D., Greenwood C., Brunori M. 1977; The electron-transfer reaction between azurin and the cytochrome c oxidase from Pseudomonas aeruginosa. Biochem J 167 447 455
    [Google Scholar]
  42. Parsonage D., Greenfield A. J., Ferguson S. J. 1986; Evidence that the energy conserving electron transport pathways to nitrate and cytochrome o branch at ubiquinone in Paracoccus denitrificans. Microbiol 145 191 196
    [Google Scholar]
  43. Richardson D. J., Bell L. C., McEwan A. G., Jackson J., Ferguson S. 1991; Cytochrome c2 is essential for electron transfer to nitrous oxide reductase from physiological substrates in Rhodobacter capsulatus and can act as an electron donor to the reductase in vitro. J Biochem 199 677 683
    [Google Scholar]
  44. Richardson D., McEwan A., Page M., Jackson J., Ferguson S. 1990; The identification of cytochromes involved in the transfer of electrons to the periplasmic nitrate reductase of Rhodobacter capsulatus and resolution of a soluble nitrate reductase-cytochrome r552 redox complex. J Biochem 194 263 270
    [Google Scholar]
  45. Robertson L., Kuenen J. 1983; Thiosphaera pantotropha gen. nov. sp. nov., facultatively anaerobic, facultatively autotrophic sulphur bacterium. Gen Microbiol 129 2847 2855
    [Google Scholar]
  46. Rott M., Witthuhn V. C, Schilke B., Soranno M., Ali A., Donohue T. 1993; Genetic evidence for the role of isocytochrome c2 in photosynthetic growth of Rhodobacter sphaeroides Spd mutants. Bacteriol 175 358 366
    [Google Scholar]
  47. Sambrook J., Fritsch E., Maniatis T. 1989; Molecular Cloning. Laboratory Manual , 2nd edn. Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  48. Sandmann G., Reck HL, Keswsler E., Boger P. 1983; Distribution of plastocyanin and soluble plastidic cytochrome c in various classes of algae. Microbiol 134 23 27
    [Google Scholar]
  49. Sawada E., Satoh T., Kitamura H. 1978; Purification and properties of a dissimilatory nitrite reductase of a denitrifying phototrophic bacterium. Cell Physiol 19 1339 1351
    [Google Scholar]
  50. Shapleigh J., Payne W. 1985; Differentiation of cytochrome cd1 and copper nitrite reductase production in denitrifiers. Microbiol Lett 26 275 279
    [Google Scholar]
  51. Simon R., Priefer U., Puhler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. j Technology 1 784 791
    [Google Scholar]
  52. Snyder S., Hollocher T. 1987; Purification and characterization of nitrous oxide reductase from Paracoccus denitrificans. Biol Chem 262 6515 6525
    [Google Scholar]
  53. van Spanning R. M., Wanseil W. M., Harms N., Ras J., Oltmann L., Stouthamer A. 1990; Mutagenesis of the gene encoding cytochrome c550 of Paracoccus denitrificans and analysis of the resultant physiological effects. Bacteriol 172 986 996
    [Google Scholar]
  54. van Spanning R. M., Wanseli C., Reijnders W. M., Harms N., Ras J., Oltmann L., Stouthamer A. 1991; A method for introduction of unmarked mutations in the genome of Paracoccus denitrificans : construction of strains with multiple mutations in the genes encoding periplasmic cytochormes c 550, c 551 and c 553 . J Bacteriol 173 6962 6970
    [Google Scholar]
  55. Steinrucke P., Gerhus E., Jetzek M., Turba A., Ludwig B. 1991; The cytochrome c reductase/oxidase respiratory pathway of Paracoccus denitrificans genetic and functional studies. Bioenerg Biomembr 23 227 239
    [Google Scholar]
  56. Stouthamer A. 1992; Metabolic pathways in Paracoccus denitrificans and closely related bacteria in relation to the phylogeny of prokaryotes. Leeuwenhoek 61 1 33
    [Google Scholar]
  57. Timkovich R., Dhesi R., Martinkus K., Robinson M., Rea T. 1982; Isolation of Paracoccus denitrificans cytochrome cd 1: comparative kinetics with other nitrite reductases. Biocbem Biophys 215 47 58
    [Google Scholar]
  58. Willison J., John P. 1979; Mutations of Paracoccus denitrificans deficient in r-type cytochromes. Gen Microbiol 115 443 450
    [Google Scholar]
  59. Yanisch-Perron C., Vieira J., Messing J. 1985 Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors 33 103 119
    [Google Scholar]
  60. Zumft W. 1992; The denitrifying prokaryotes. The Prokaryotes , 2nd edn. vol. 1 Edited by Balows A., Trüper H. G., Dworkin W., Harder K.-H. Berlin: Springer Verlag;
    [Google Scholar]
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