1887

Abstract

Summary: A mutant of has been isolated by continuous selection in increasing concentrations of HO. It grew with a doubling time of 85 min in minimal medium containing 150 mM HO, whereas the wild-type parent lysed in 100 mM HO. The mutant was also more resistant to organic peroxides than the wild-type. Further resistance to HO could not be induced by pretreatment with low concentrations of the oxidant. The mutant synthesized a number of proteins at a much higher rate than the wild-type, including constitutive synthesis of all of the proteins which were induced by HO in the wild-type. Four of these proteins were sequenced; three were identified as catalase and two subunits of alkyl hydroperoxide reductase. Two proteins whose synthesis was repressed in the mutant were sequenced, and one was identified as flagellin. The mutant grew as non-flagellated, partially septate, filaments of cells, and fragments of flagella were seen in the surrounding medium.

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1994-02-01
2021-07-29
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References

  1. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105 121 126
    [Google Scholar]
  2. Bol D. K., & Yasbin R. E. Characterization of an inducible oxidative stress system in Bacillus subtilis. J Bacteriol 1990; 172 3503 3506
    [Google Scholar]
  3. Bol D. K., & Yasbin R. E. The isolation, cloning and identification of a vegetative catalase gene from Bacillus subtilis. Gene 1991; 109 31 37
    [Google Scholar]
  4. Christman M. K., , Morgan R. W., , Jacobson F. S., & Ames B. N. Positive control of a regulon for defences against oxidative stress and some heat-shock proteins in Salmonella typhimurium. Cell 1985; 41 753 762
    [Google Scholar]
  5. Darr D., , Zarilla K. A., & Fridovich I. A mimic of superoxide dismutase activity based upon desferrioxamine B and manganese (IV). Arch Biochem Biophys 1987; 258 351 355
    [Google Scholar]
  6. DeLange R. J., , Chang J. Y., , Shaper J. H., & Glazer A. N. Amino acid sequence of flagellin of Bacillus subtilis 168. J Biol Chem 1976; 251 705 711
    [Google Scholar]
  7. Demple B., & Halbrook J. Inducible repair of oxidative DNA damage in Escherichia coli. Nature 1983; 304 466 468
    [Google Scholar]
  8. Donovan W., Zheng L., Sandman K., Losick R. 1987; Genes encoding spore coat polypeptides from Bacillus subtilis. J Mol Biol 196 1 10
    [Google Scholar]
  9. Dowds B. C. A., Hoch J. A. Regulation of the oxidative stress response by the hpr gene in Bacillus subtilis. J Gen Microbiol 1991; 137 1121 1125
    [Google Scholar]
  10. Dowds B. C. A., , Murphy P., , McConnell D., & Devine K. M. Relationship among oxidative stress, growth cycle, and sporulation in Bacillus subtilis. J Bacteriol 1987; 169 5771 5775
    [Google Scholar]
  11. Farr S. B., Kogoma T. Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol Rev 1991; 55 561 585
    [Google Scholar]
  12. Goldberg I., & Mekalanos J. J. Effect of a recA mutation on cholera toxin gene amplification and deletion events. J Bacteriol 1986; 165 723 731
    [Google Scholar]
  13. Greenberg J. T., Demple B. A global response induced in Escherichia coli by redox-cycling agents overlaps with that induced by peroxide stress. J Bacteriol 1989; 171 3933 3939
    [Google Scholar]
  14. Hartford O., Dowds B. C. A. 1992; Cloning and characterization of genes induced by hydrogen peroxide in Bacillus subtilis. J Gen Microbiol 138 2061 2068
    [Google Scholar]
  15. Helmann J. D., Marquez L. M., Chamberlin M. J. 1988; Cloning, sequencing, and disruption of the Bacillus subtilis ct28 gene. J Bacteriol 170 1568 1574
    [Google Scholar]
  16. Hornemann U., , Otto C. J., , Hoffman G. G., Bertinuson A. C. Spectinomycin resistance and associated DNA amplification in Streptomyces achromogenes subsp rubradiris. J Bacteriol 1987; 169 2360 2366
    [Google Scholar]
  17. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 1987; 262 10035 10038
    [Google Scholar]
  18. Murphy P., , Dowds B. C. A., , McConnell D. J., Devine K. M. Oxidative stress and growth temperature in Bacillus subtilis. J Bacteriol 1987; 169 5766 5770
    [Google Scholar]
  19. Nicholson W. L., Setlow P. Sporulation, germination and outgrowth. In Molecular Biological Methods for Bacillus 1990 Edited by Harwood C. R., Cutting S. M. Chichester: John Wiley; pp 391 450
    [Google Scholar]
  20. Sambrook J., , Fritsch E. F., Maniatis T. Cold Spring Harbor. Molecular Cloning: A Eaboratory Manual , 2nd edn. 1989 NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Sauret G., Jouve H., Pelmont J. 1979; Proprietes d’un mutant de Proteus mirabilis resistant au peroxyde d’hydrogene. Canadian J Microbiol 25 312 320
    [Google Scholar]
  22. Schaeffer P., Miller T., Aubert J. 1965; Catabolic repression of bacterial sporulation. Proc Natl Acad Sci USA 54 701 711
    [Google Scholar]
  23. Storz G., Jacobson F. S., Tartaglia L. A., Morgan R. W., Silveira L. A., Ames B. N. An alkyl hydroperoxide reductase induced by oxidative stress in Salmonella typhimurium and Escherichia coli genetic characterization and cloning of ahp. J Bacteriol 1989; 171 2049 2055
    [Google Scholar]
  24. Storz G., Tartaglia L. A., Ames B. N. Transcriptional regulator of oxidative stress-inducible genes: direct activation of oxidation. Science 1990; 248 189 194
    [Google Scholar]
  25. Tartaglia L. A., Storz G., Brodsky M. H., Lai A., Ames B. N. 1990; Alkyl hydroperoxide reductase from Salmonella typhimurium. J Biol Chem 265 10535 10540
    [Google Scholar]
  26. Trach K., Burbulys D., Spiegelman G., Perego M., Van Hoy B., Strauch M., Day J., Hoch J. A. Phosphorylation of the SpoOA protein: a cumulative environsensory activation mechanism. In Genetics and Biotechnology of Bacilli 1990 vol 3 Edited by Zukowski M. M., Ganesan A. T., Hoch J. A. San Diego: Academic Press; pp 357 365
    [Google Scholar]
  27. Wu J., Weiss B. Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of Escherichia coli. J Bacteriol 1991; 173 2864 2871
    [Google Scholar]
  28. Yasbin R. E., Fields P. I., Anderson B. J. Properties of Bacillus subtilis 168 derivatives freed of their natural prophages. Gene 1980; 12 155 159
    [Google Scholar]
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