Protoplasts were prepared from two auxotrophic and antibiotic-resistant strains (MetNaland ThrStr, respectively) of the facultative alkaliphile sp. C-125 by treatment with lysozyme. The protoplasts fused effectively in the presence of polyethylene glycol. Fusants obtained between two parental protoplasts were regenerated on solid medium containing the two antibiotics, nalidixic acid (Nal) and streptomycin (Str). Parental protoplasts were regenerated at high frequency (43-97%) on non-selective medium but not on selective medium. The NalStrfusants had the form of bacilli. Met and Thr markers segregated among the fusants with a predominantly MetThrphenotype. The exfusants seemed to restore the parental ploidy.


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