1887

Abstract

Catransport has been measured across plasma membrane vesicles isolated from cells of . This transport was found to be ATP- (or to a lesser extent GTP) and Mg-dependent. Inconsistent release of Cafrom intact vesicles was obtained using the calcium ionophore A23187. However, Cawas released by Triton X-100 in a concentration-dependent manner. Transport was inhibited by vanadate (>50%) and erythrosin B (about 50%), being about 10 μM for both inhibitors. In the presence of the protonophores CCCP or gramicidin, partial inhibition of Catransport (about 20%) was observed, but the Ca-channel blockers, nifedipine, diltiazem and verapamil had no effect, although the latter inhibited proton transport. The results indicate that Catransport in is regulated by a P-type ATPase with similar properties to that found in higher plants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-11-3047
1994-11-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/11/mic-140-11-3047.html?itemId=/content/journal/micro/10.1099/13500872-140-11-3047&mimeType=html&fmt=ahah

References

  1. Berridge M.J. Inositol trisphosphate and calcium signalling. Nature 1993; 361:315–325
    [Google Scholar]
  2. Bradford M.M. A rapid and sensitive method for the quantitation of submicrogram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248–254
    [Google Scholar]
  3. Carnelli A., De Michelis M.I., Rasi-Caldogno F. Plasma membrane Ca-ATPase of radish seedlings. 1. Biochemical characterization using ITP as substrate. Plant Physiol 1992; 102:651–661
    [Google Scholar]
  4. Clarkson D.T., Brownlee C., Ayling S.M. Cytoplasmic calcium measurements in intact higher plant cells: results from fluorescence ratio imaging of fura-2. J Cell Sci 1988; 91:71–80
    [Google Scholar]
  5. Coupland D., Cooke D.T., James C.S. Effects of 4-chloro-2-methylphenoxypropionate (an auxin analogue) on plasma membrane ATPase activity in herbicide-resistant and herbicide-susceptible biotypes of Stellaria media L. J Exp Bot 1991; 42:1065–1071
    [Google Scholar]
  6. Dawson R.M.C., Elliot W.H., Jones K.M. Data for Biochemical Research 1986 Oxford: Oxford Science Publications;
    [Google Scholar]
  7. Evans D.E., Briars S.A., Williams L.E. Active calcium transport by plant cell membranes. J Exp Bot 1991; 42:285–303
    [Google Scholar]
  8. Frazer L.N., Moore D. Antagonists and inhibitors of calcium accumulation do not impair gravity perception though they adversely affect the gravitropic responses of Coprinus cinereus stipes. Mycol Res 1993; 97:113–118
    [Google Scholar]
  9. Giannini J.A., Holt J.S., Briskin D.P. Isolation of sealed plasma membranes from Phytophtora megasperma f.sp. glycinea: II Partial characterization of Ca2+transport and glyceollin effects.. Arch Biochem Biophys 1988; 266:644–649
    [Google Scholar]
  10. Halachmi D., Ghislain M., Eilam Y. An intracellular ATP-dependent calcium pump within the yeast Schizosaccharomyces pombe, encoded by the gene cta3. Eur J Biochem 1992; 207:1003–1008
    [Google Scholar]
  11. Hargreaves J.A., Turner G. Gene transformation in plant pathogenic fungi. In Molecular Plant Pathology - A Practical Approach 1992 Edited by Gurr S.J., McPherson J.M., Bowles D.J. Oxford and Washington D.C.: IRL Press; 1 pp 79–96
    [Google Scholar]
  12. Jackson S.L., Heath I.B. Roles of calcium in hyphal tip growth. Microbiol Rev 1993; 57:367–382
    [Google Scholar]
  13. Larsson C., Widell S., Kjellbom P. Preparation of high purity plasma membranes. Methods Engymol 1987; 148:558–568
    [Google Scholar]
  14. Martell A.E., Smith R.M. Critical Stability Constants 1982; 5 First Supplement New York: Plenum Press;
    [Google Scholar]
  15. Miller A.J., Vogg G., Sanders D. Cytosolic calcium homeostasis in fungi: dual roles of plasma membrane transport and intracellular sequestration of calcium. Proc Natl Acad Sci USA 1990; 87:9348–9352
    [Google Scholar]
  16. Okorokov L.A., Tanner W., Lehle L. A novel primary Ca2+-transport system from Saccharomyces cerevisiae. Eur J Biochem 1993; 216:573–577
    [Google Scholar]
  17. Pitt D., Barnes J.C. Calcium homeostasis, signalling and protein phosphorylation during calcium-induced conidiation in Penicillium notatum. J Gen Microbiol 1993; 139:3053–3063
    [Google Scholar]
  18. Ramón A.M., Cooke D.T., Clarkson D.T. Calcium transport across plasma membrane vesicles isolated from shoots of Stellaria media and Avena sativa. Physiol Plant 1993; 89:731–740
    [Google Scholar]
  19. Scarborough G.A. Isolation of everted plasma membrane vesicles from Neurospora crassa and measurement of transport function. Methods Engymol 1989; 174:667–676
    [Google Scholar]
  20. Stroobant P., Scarborough G.A. Active transport of calcium in Neurospora plasma membranes. Proc Natl Acad Sci USA 1979; 76:3102–3106
    [Google Scholar]
  21. Tada M., Kadoma M., Inui M., Fujii J.I. Regulation of Ca2+-pump from cardiac sarcoplasmic reticulum. Methods Engymol 1988; 157:109
    [Google Scholar]
  22. Williams L.E., Schueler S.B., Briskin D.P. Further characterization of the red beet plasma membrane Ca2+ATPase using GTP as an alternative substrate. Plant Physiol 1990; 92:747–754
    [Google Scholar]
  23. Wimmers L.E., Ewing N.N., Bennett A.B. Higher plant Ca2+-ATPase: primary structure and regulation of mRNA abundance by salt. Proc Natl Acad Sci USA 1992; 89:9205–9209
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-11-3047
Loading
/content/journal/micro/10.1099/13500872-140-11-3047
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error