1887

Abstract

Summary: Extant K12 strains are phenotypically rough, their lipopolysaccharide having a complete core structure, but no O antigen. We used DNA hybridization and DNA sequencing to show that the rough phenotype of this strain is due to the presence of one of two independent mutations in the gene cluster. The mutation, consisting of an IS5 insertion at the downstream end of , is present in strain EMG2, which is representative of most K12 derivatives. The mutation is a deletion at the upstream end of , and was found in strain WG1. A gene cloned from strain WG1 could complement the mutation in strain EMG2, and the complemented strain produced O antigen which was typed as O16 with cross reaction to O17.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-1-49
1994-01-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/1/mic-140-1-49.html?itemId=/content/journal/micro/10.1099/13500872-140-1-49&mimeType=html&fmt=ahah

References

  1. Al-Hendy A., Toivanen P., Skurnik M. Expression and cloning of Yersinia enterocolitica O:3 rfb gene cluster in Escherichia coli K12. Microb Pathog 1991; 10:47–59
    [Google Scholar]
  2. Altschul A. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol 1990; 215:403–410
    [Google Scholar]
  3. Appleyard R. K. Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K-12. Genetics 1953; 39:440–452
    [Google Scholar]
  4. Appleyard R. K., McGregor J. F., Baird K. M. Mutation to extended host range and the occurrence of phenotypic mixing in the temperate coliphage. Virology 1956; 2:565–574
    [Google Scholar]
  5. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 1972; 36:525–557
    [Google Scholar]
  6. Bachmann B. J. Linkage map of Escbericbia coli K-12, edition 7. Microbiol Rev 1983; 47:180–230
    [Google Scholar]
  7. Bachmann B. J. Derivations and genotypes of some mutant derivatives of Escherichia coli K12. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology Edited by Neidhardt F.C., Ingraham J.L., Low K.B., Magasanik B., Schaechter M., Umbarger H.E. Washington, DC: American Society for Microbiology.; 1987; 21190–1219
    [Google Scholar]
  8. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev 1990; 54:130–197
    [Google Scholar]
  9. Barcak G. J., Wolf R. E.,Jr. Growth-rate-dependent expression and cloning of gnd alleles from natural isolates of Escherichia coli. J Bacteriol 1988; 170:365–371
    [Google Scholar]
  10. Bastin D. A., Romana L. K., Reeves P. R. Molecular cloning and expression in Escherichia coli K-12 of the rfl gene cluster determining the 0 antigen of an E. coli 0111 strain. Mol Microbiol 1991; 5:2223–2231
    [Google Scholar]
  11. Bouffard G., Ostell J., Rudd K. E. GeneScape: a relational database of Escherichia coli genomic map data for Macintosh computers. Comput Applic Biosci 1992; 8:563–567
    [Google Scholar]
  12. Brown P. K., Romana L. K., Reeves P. R. Cloning of the rfb gene cluster of a group C2 Salmonella strain: comparison with rfb regions of groups B and D. Mol Microbiol 1991; 5:1873–1881
    [Google Scholar]
  13. Calos M. P., Miller J. H. Transposable elements. Cell 1980; 20:579–595
    [Google Scholar]
  14. Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol 1978; 47:45–148
    [Google Scholar]
  15. Clowes R. C., Rowley D. Some observations on linkage effects in genetic recombination in Escherichia coli K-12. J Gen Microbiol 1954; 11:250–260
    [Google Scholar]
  16. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 1984; 12:387–395
    [Google Scholar]
  17. Eisenberg D., Schwar E., Komaromy M., Wall R. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol 1984; 179:125–142
    [Google Scholar]
  18. Engler J. A., van Bree M. P. The nucleotide sequence and protein-coding capability of the transposable element IS5. Gene 1981; 14:155–163
    [Google Scholar]
  19. Frischauf A.-M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. I Mol Biol 1983; 170:827–842
    [Google Scholar]
  20. Gish W., States D. J. Identification of protein coding regions by database similarity search. Nature Genet 1993; 3:266–272
    [Google Scholar]
  21. Gray C. H., Tatum E. L. X-ray induced growth factor deficiencies in bacteria. Proc Natl Acad Sci USA 1944; 30:404–410
    [Google Scholar]
  22. Hayes w. Observations on a transmissible agent determining sexual differentiation in Bacterium coli. J Gen Microbiol 1953; 8:72–88
    [Google Scholar]
  23. Jiang X. M., Neal B., Santiago F., Lee S. J., Romana L. K., Reeves P. R. Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Mol Microbiol 1991; 5:695–713
    [Google Scholar]
  24. Klein P., Kanehisa M., Delisi C. The detection and classification of membrane spanning proteins. Biochim Biophys Acta 1985; 815:468–476
    [Google Scholar]
  25. Klena J. D., Schaitman J. D. Function of the rfb gene cluster and the rfb gene in the synthesis of O antigen by Shigella dysenteriae 1. Mol Microbiol 1993; 9:393–402
    [Google Scholar]
  26. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 1987; 50:495–508
    [Google Scholar]
  27. Lederberg E. M., Lederberg J. Genetic studies of lysogenicity in Escherichia coli. Genetics 1953; 38:51–64
    [Google Scholar]
  28. Lederberg J., Tatum L. Gene recombination in Escherichia coli. Nature 1946; 158:558
    [Google Scholar]
  29. Low B. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J Bacteriol 1973; 113:798–812
    [Google Scholar]
  30. Miller R. D., Dykhuizen D. E., Hartl D. L. Fitness effects of a deletion mutation increasing transcription of the 6-phospho- gluconate dehydrogenase gene in Escherichia coli. Mol Biol Evol 1988; 5:691–703
    [Google Scholar]
  31. Nasoff M. S., Wolf R. E., Jr. Molecular cloning, correlation of genetic and restriction maps, and determination of the direction of transcription of gnd of Escherichia coli. J Bacteriol 1980; 143:731–741
    [Google Scholar]
  32. Nasoff M. S., Baker H. V., II., Wolf R. E., Jr. DNA sequence of the Escherichia coli gene, gnd, for 6-phosphogluconate dehydrogenase. Gene 1984; 27:253–264
    [Google Scholar]
  33. Neuhard J., Thomassen E. Altered deoxyribonucleotide pools in P2 educants of Escherichia coli K-12 due to deletion of the dcd gene. J Bacteriol 1976; 126:999–1001
    [Google Scholar]
  34. Nikaido H., Nikaido K., Rapin A. M. C. Biosynthesis of thymidine diphosphate L-rhamnose in Escherichia coli K-12. Biochim Biophys Acta 1965; 3:548–551
    [Google Scholar]
  35. Prehm P., Schmidt G., Jann B. The cell-wall lipopolysaccharide of Escherichia coli K-12. Eur J Biochem 1976; 70:171–177
    [Google Scholar]
  36. Reeves P. R. Variation in O antigens, niche specific selection and bacterial populations. FEMS Microbiol Lett 1992; 100:509–516
    [Google Scholar]
  37. Reisner A. H., Bucholtz C. A., Smelt J., McNeil S. Australian national genetic information system. Proceedings of the 26th Annual Hawaii International Conference in Systems Sciences Edited by Mudge T.N., Milutinovic V., Munter L. Los Alamos: IEEE Computer Society; 1993; 1595–602
    [Google Scholar]
  38. Rudd K. E., Bouffard G., Miller W. Computer analysis of E. coli restriction map. Genome Analysis Edited by Davies K.E., Tilghman S.M. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1993; 4:1–38
    [Google Scholar]
  39. Saiki R. K., Gelfand D. H., Stofell S., Scharf S. J., Higuchi R., Horn G.T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988; 239:487–491
    [Google Scholar]
  40. Sambrook J., Fritsch E. F., Maniati T. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. Molecular Cloning: a Laboratory Manual 1989
    [Google Scholar]
  41. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 1977; 74:5463–5467
    [Google Scholar]
  42. Schmidt G. Genetical studies on the lipopolysaccharide structure of Escherichia coli K12. J Gen Microbiol 1973; 77:157–160
    [Google Scholar]
  43. Stevenson G., Lee S. J., Romana L. K. The cps gene cluster of Salmonella strain LT2 includes a second mannose pathway: sequence of two genes and relationship to genes in the rfb gene cluster.. Mol & Gen Genet 1991; 227:173–180
    [Google Scholar]
  44. Tatum E. L., Lederberg J. Gene recombination in bacterium Escherichia coli. J Bacteriol 1947; 53:673–684
    [Google Scholar]
  45. Valvano M. A., Crosa J. H. Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O7 lipopolysaccharide antigen of a human invasive strain of E. coli O7:K1. . Infect Immun 1989; 157:937–943
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-1-49
Loading
/content/journal/micro/10.1099/13500872-140-1-49
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error